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Published ahead of print on November 11, 2004, doi:10.1165/rcmb.2004-0239OC
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American Journal of Respiratory Cell and Molecular Biology. Vol. 32, pp. 142-148, 2005
© 2005 American Thoracic Society
DOI: 10.1165/rcmb.2004-0239OC

Characterization of the Interstitial Lung and Peripheral Blood T Cell Receptor Repertoire in Cigarette Smokers

Stephanie Korn, Rainer Wiewrodt, Yvonne C. Walz*, Kai Becker, Eckhard Mayer, Frank Krummenauer and Roland Buhl

Pulmonary Department, Department for Medical Biometry, Epidemiology and Informatics, and Department of Cardiothoracic and Vascular Surgery, Mainz University Hospital, Mainz, Germany

Correspondence and requests for reprints should be addressed to Roland Buhl, Pulmonary Department, Mainz University Hospital, Langenbeckstrasse 1, D-55131 Mainz, Germany. E-mail: r.buhl{at}3-med.klinik.uni-mainz.de

T lymphocytes modulate the pulmonary inflammatory response. The aim of this study was to evaluate the clonality within the interstitial lung and peripheral blood T cell receptor (TCR) repertoire in smokers. Interstitial T lymphocytes were isolated from surplus tissue of 16 patients (63 ± 9 [± SD] yr old, 11 male) undergoing surgery due to lung cancer (n = 15) or emphysema. TCR clonality was assessed by PCR amplification followed by spectratyping. Nearly all TCR of interstitial lung lymphocytes showed oligoclonal bands (CD4+ subset 13/16 patients, 81%; CD8+ 100%) indicating a specific differentiation. Peripheral blood T lymphocytes (PBL) TCR (especially CD4+) had less oligoclonal bands (CD4+ 31%, CD8+ 88%). Likewise, more oligoclonal bands were seen in lung TCR (total of 168 bands; 37 CD4+; 131 CD8+), compared with 59 bands in PBL TCR (13 CD4+; 46 CD8+). Intraindividual comparison revealed a more prominent difference in TCR oligoclonality between lung and blood in CD8+ T cells (median of difference lung minus blood 5; interquartile range 1–10; P = 0.002) compared with CD4+ T cells (median 2, 0–3, P = 0.039). Thus, TCR oligoclonality is preferentially found in the CD8+ T cell subset, most distinctive in the lung. These findings indicate a specific interstitial T cell differentiation in response to local stimuli.

Key Words: smoker • lymphocytes • T cell receptor • lung • interstitial compartment




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