help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

Published ahead of print on November 11, 2004, doi:10.1165/rcmb.2004-0239OC
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2004-0239OCv1
32/2/142    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Korn, S.
Right arrow Articles by Buhl, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Korn, S.
Right arrow Articles by Buhl, R.
American Journal of Respiratory Cell and Molecular Biology. Vol. 32, pp. 142-148, 2005
© 2005 American Thoracic Society
DOI: 10.1165/rcmb.2004-0239OC

Characterization of the Interstitial Lung and Peripheral Blood T Cell Receptor Repertoire in Cigarette Smokers

Stephanie Korn, Rainer Wiewrodt, Yvonne C. Walz*, Kai Becker, Eckhard Mayer, Frank Krummenauer and Roland Buhl

Pulmonary Department, Department for Medical Biometry, Epidemiology and Informatics, and Department of Cardiothoracic and Vascular Surgery, Mainz University Hospital, Mainz, Germany

Correspondence and requests for reprints should be addressed to Roland Buhl, Pulmonary Department, Mainz University Hospital, Langenbeckstrasse 1, D-55131 Mainz, Germany. E-mail: r.buhl{at}3-med.klinik.uni-mainz.de

T lymphocytes modulate the pulmonary inflammatory response. The aim of this study was to evaluate the clonality within the interstitial lung and peripheral blood T cell receptor (TCR) repertoire in smokers. Interstitial T lymphocytes were isolated from surplus tissue of 16 patients (63 ± 9 [± SD] yr old, 11 male) undergoing surgery due to lung cancer (n = 15) or emphysema. TCR clonality was assessed by PCR amplification followed by spectratyping. Nearly all TCR of interstitial lung lymphocytes showed oligoclonal bands (CD4+ subset 13/16 patients, 81%; CD8+ 100%) indicating a specific differentiation. Peripheral blood T lymphocytes (PBL) TCR (especially CD4+) had less oligoclonal bands (CD4+ 31%, CD8+ 88%). Likewise, more oligoclonal bands were seen in lung TCR (total of 168 bands; 37 CD4+; 131 CD8+), compared with 59 bands in PBL TCR (13 CD4+; 46 CD8+). Intraindividual comparison revealed a more prominent difference in TCR oligoclonality between lung and blood in CD8+ T cells (median of difference lung minus blood 5; interquartile range 1–10; P = 0.002) compared with CD4+ T cells (median 2, 0–3, P = 0.039). Thus, TCR oligoclonality is preferentially found in the CD8+ T cell subset, most distinctive in the lung. These findings indicate a specific interstitial T cell differentiation in response to local stimuli.

Key Words: smoker • lymphocytes • T cell receptor • lung • interstitial compartment




This article has been cited by other articles:


Home page
Eur Respir JHome page
B. Barcelo, J. Pons, J. M. Ferrer, J. Sauleda, A. Fuster, and A. G. N. Agusti
Phenotypic characterisation of T-lymphocytes in COPD: abnormal CD4+CD25+ regulatory T-lymphocyte response to tobacco smoking
Eur. Respir. J., March 1, 2008; 31(3): 555 - 562.
[Abstract] [Full Text] [PDF]


Home page
Physiol. Rev.Home page
T. Yoshida and R. M. Tuder
Pathobiology of Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease
Physiol Rev, July 1, 2007; 87(3): 1047 - 1082.
[Abstract] [Full Text] [PDF]


Home page
Proc Am Thorac SocHome page
R. M. Tuder, T. Yoshida, W. Arap, R. Pasqualini, and I. Petrache
State of the Art. Cellular and Molecular Mechanisms of Alveolar Destruction in Emphysema: An Evolutionary Perspective
Proceedings of the ATS, August 1, 2006; 3(6): 503 - 510.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Crit. Care Med.Home page
D. Rosen, J.-H. Lee, F. Cuttitta, F. Rafiqi, S. Degan, and M. E. Sunday
Accelerated Thymic Maturation and Autoreactive T Cells in Bronchopulmonary Dysplasia
Am. J. Respir. Crit. Care Med., July 1, 2006; 174(1): 75 - 83.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. Vlahos, S. Bozinovski, J. E. Jones, J. Powell, J. Gras, A. Lilja, M. J. Hansen, R. C. Gualano, L. Irving, and G. P. Anderson
Differential protease, innate immunity, and NF-{kappa}B induction profiles during lung inflammation induced by subchronic cigarette smoke exposure in mice
Am J Physiol Lung Cell Mol Physiol, May 1, 2006; 290(5): L931 - L945.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Crit. Care Med.Home page
A. K. Sullivan, P. L. Simonian, M. T. Falta, J. D. Mitchell, G. P. Cosgrove, K. K. Brown, B. L. Kotzin, N. F. Voelkel, and A. P. Fontenot
Oligoclonal CD4+ T Cells in the Lungs of Patients with Severe Emphysema
Am. J. Respir. Crit. Care Med., September 1, 2005; 172(5): 590 - 596.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2005 American Thoracic Society.