help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Published ahead of print on April 28, 2005, doi:10.1165/rcmb.2004-0347OC
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Online Supplement
Right arrow All Versions of this Article:
2004-0347OCv1
33/2/178    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Demoule, A.
Right arrow Articles by Petrof, B. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Demoule, A.
Right arrow Articles by Petrof, B. J.
American Journal of Respiratory Cell and Molecular Biology. Vol. 33, pp. 178-185, 2005
© 2005 American Thoracic Society
DOI: 10.1165/rcmb.2004-0347OC

Expression and Regulation of CC Class Chemokines in the Dystrophic (mdx) Diaphragm

Alexandre Demoule, Maziar Divangahi, Gawiyou Danialou, Dusanka Gvozdic, Gary Larkin, Weisheng Bao and Basil J. Petrof

Meakins-Christie Laboratories, McGill University, Montreal; and Respiratory Division, McGill University Health Centre, Montreal, Quebec, Canada

Correspondence and requests for reprints should be addressed to Basil J. Petrof, M.D., Respiratory Division, Room L411, Royal Victoria Hospital, 687 Pine Ave. West, Montreal, H3A 1A1, PQ Canada. E-mail: basil.petrof{at}mcgill.ca

In the murine (mdx) model of Duchenne muscular dystrophy, dystrophic changes are much more severe in the diaphragm than in limb muscles, and the diaphragm more closely resembles the human disease phenotype. Chemokines could play a central role in governing such phenotypic differences, as inflammation is an important disease modifier. Here we report that CC chemokine receptors (CCRs 1, 2, 3, 5) and ligands (macrophage inflammatory protein-1{alpha}, RANTES) are expressed at higher levels in dystrophic than in wild-type muscles across age groups (6, 12, and 24 wk). Moreover, chemokine ligand expression and muscle inflammation are significantly higher in dystrophic diaphragms than in limb muscles of the same animals. In vitro, CCR1 is constitutively expressed by cultured primary diaphragmatic myotubes. Stimulation of myotubes by proinflammatory cytokines (tumor necrosis factor-{alpha}, interleukin-1{alpha}, interferon-{gamma}) found within the in vivo dystrophic muscle environment, upregulates CCR1 in mdx and wild-type cultures, and also increases expression of its ligand RANTES to a significantly greater degree in the mdx group. Taken together, our results suggest that CC chemokines may play an important role in sustaining inflammation within the mdx diaphragm, which could help acount for its more severe phenotype and also offer a target for therapeutic intervention in Duchenne patients.

Key Words: chemokine receptors • Duchenne muscular dystrophy • inflammation • macrophage inflammatory protein-1{alpha} • RANTES • skeletal muscle




This article has been cited by other articles:


Home page
 J. Physiol.Home page
L. Yahiaoui, D. Gvozdic, G. Danialou, M. Mack, and B. J. Petrof
CC family chemokines directly regulate myoblast responses to skeletal muscle injury
J. Physiol., August 15, 2008; 586(16): 3991 - 4004.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Divangahi, T. Yang, K. Kugathasan, S. McCormick, S. Takenaka, G. Gaschler, A. Ashkar, M. Stampfli, J. Gauldie, J. Bramson, et al.
Critical Negative Regulation of Type 1 T Cell Immunity and Immunopathology by Signaling Adaptor DAP12 during Intracellular Infection
J. Immunol., September 15, 2007; 179(6): 4015 - 4026.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Divangahi, A. Demoule, G. Danialou, L. Yahiaoui, W. Bao, Z. Xing, and B. J. Petrof
Impact of IL-10 on Diaphragmatic Cytokine Expression and Contractility during Pseudomonas Infection
Am. J. Respir. Cell Mol. Biol., April 1, 2007; 36(4): 504 - 512.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. H. Boyd, M. Divangahi, L. Yahiaoui, D. Gvozdic, S. Qureshi, and B. J. Petrof
Toll-Like Receptors Differentially Regulate CC and CXC Chemokines in Skeletal Muscle via NF-{kappa}B and Calcineurin
Infect. Immun., December 1, 2006; 74(12): 6829 - 6838.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Demoule, M. Divangahi, L. Yahiaoui, G. Danialou, D. Gvozdic, K. Labbe, W. Bao, and B. J. Petrof
Endotoxin Triggers Nuclear Factor-{kappa}B-dependent Up-regulation of Multiple Proinflammatory Genes in the Diaphragm
Am. J. Respir. Crit. Care Med., September 15, 2006; 174(6): 646 - 653.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2005 American Thoracic Society. 		  Work-Life