help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Published ahead of print on June 9, 2005, doi:10.1165/rcmb.2005-0067OC
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2005-0067OCv1
33/2/203    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hammerschmidt, S.
Right arrow Articles by Wirtz, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hammerschmidt, S.
Right arrow Articles by Wirtz, H.
American Journal of Respiratory Cell and Molecular Biology. Vol. 33, pp. 203-210, 2005
© 2005 American Thoracic Society
DOI: 10.1165/rcmb.2005-0067OC

Mechanical Stretch Alters Alveolar Type II Cell Mediator Release toward a Proinflammatory Pattern

Stefan Hammerschmidt*, Hartmut Kuhn*, Ulrich Sack, Anke Schlenska, Christian Gessner, Adrian Gillissen and Hubert Wirtz

Department of Respiratory Medicine and Critical Care, and Department of Immunology, University of Leipzig; and Robert-Koch-Klinik, Leipzig, Germany

Correspondence and requests for reprints should be addressed to PD Dr. Stefan Hammerschmidt, Medizinische Universitätsklinik I, Pneumologie, Universität Leipzig, Johannisallee 32, 04103 Leipzig, Germany. E-mail: stefan.hammerschmidt{at}t-online.de

Increased mechanical stretch of alveolar type II (ATII) cells occurs during mechanical ventilation. The effects of three patterns of stretching rat ATII cells (frequency [min-1]-{Delta}surface area [%]: S40-13, S60-13, S40-30) were compared with those in static cultures at 12, 18, and 24 h. Cell viability and expression of cyclooxygenase-2,5-lipoxygenase, inducible nitric oxide synthase (iNOS), and endothelial nitric oxide synthase (eNOS) were characterized. Supernatants were analyzed for eicosanoids, nitrite, cytokines, and stimulatory effects on rat lymphocytes. S40-13 simulates normal breathing; the other patterns increased amplitude and frequency. There were no significant differences between S40-13 and static cultures. S60-13 only significantly increased the supernatant nitrite (11.2 ± 1.6 versus 3.9 ± 0.4 µM at 24 h). S40-30 significantly reduced the number of trypan blue–excluding cells, increased the supernatant concentration of TXB2 (4.1 ± 0.61 versus 2.2 ± 0.36 pg/ml), 6-keto-PGF1{alpha} (8.7 ± 1.0 versus 6.7 ± 0.52 pg/ml), cysteinyl-LT (12.2 ± 2.0 versus 6.1 ± 0.75 pg/ml) and nitrite (7.2 ± 1.7 versus 3.9 ± 0.4 µM). S40-30 did not alter the release of tumor necrosis factor-{alpha} and monocyte chemotactic protein-1, but significantly reduced the concentration of the anti-inflammatory interleukin-10 (20.8 ± 13.3 versus 130 ± 21.5 pg/ml). Expression of cyclooxygenase-2/5-lipoxygenase was increased/decreased; expression of iNOS/eNOS was unchanged by high-amplitude stretch. Supernatants from S40-30 experiments caused lymphocyte activation measured by CD71 and CD54 surface expression. Continuing mechanical distension of ATII cells contributes to an inflammatory response by a shift in the balance of pro- and anti-inflammatory mediators.

Key Words: alveolar type II cell • mechanical stretch • mediator release




This article has been cited by other articles:


Home page
 Br J AnaesthHome page
A. Kozian, T. Schilling, F. Freden, E. Maripuu, C. Rocken, C. Strang, T. Hachenberg, and G. Hedenstierna
One-lung ventilation induces hyperperfusion and alveolar damage in the ventilated lung: an experimental study
Br. J. Anaesth., April 1, 2008; 100(4): 549 - 559.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H.-S. Lee, Y. Wang, B. S. Maciejewski, K. Esho, C. Fulton, S. Sharma, and J. Sanchez-Esteban
Interleukin-10 protects cultured fetal rat type II epithelial cells from injury induced by mechanical stretch
Am J Physiol Lung Cell Mol Physiol, February 1, 2008; 294(2): L225 - L232.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
O. R. Rana, C. Zobel, E. Saygili, K. Brixius, F. Gramley, T. Schimpf, K. Mischke, D. Frechen, C. Knackstedt, R. H. G. Schwinger, et al.
A simple device to apply equibiaxial strain to cells cultured on flexible membranes
Am J Physiol Heart Circ Physiol, January 1, 2008; 294(1): H532 - H540.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. A. Mohammed, N. Nasreen, R. S. Tepper, and V. B. Antony
Cyclic stretch induces PlGF expression in bronchial airway epithelial cells via nitric oxide release
Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L559 - L566.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. M. McAdams, S. B. Mustafa, J. S. Shenberger, P. S. Dixon, B. M. Henson, and R. J. DiGeronimo
Cyclic stretch attenuates effects of hyperoxia on cell proliferation and viability in human alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2006; 291(2): L166 - L174.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2005 American Thoracic Society.