help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Published ahead of print on June 16, 2005, doi:10.1165/rcmb.2005-0129OC
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2005-0129OCv1
33/4/335    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chang, J. C.
Right arrow Articles by Fine, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chang, J. C.
Right arrow Articles by Fine, A.
American Journal of Respiratory Cell and Molecular Biology. Vol. 33, pp. 335-342, 2005
© 2005 American Thoracic Society
DOI: 10.1165/rcmb.2005-0129OC

Evidence that Bone Marrow Cells Do Not Contribute to the Alveolar Epithelium

Jacqueline C. Chang, Ross Summer, Xi Sun, Kathleen Fitzsimmons and Alan Fine

The Pulmonary Center, Boston University School of Medicine, Boston, Massachusetts

Correspondence and requests for reprints should be addressed to Jacqueline C. Chang, The Pulmonary Center, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118. E-mail: jachang{at}lung.bumc.bu.edu

An ongoing controversy is the role of marrow cells in populating the alveolar epithelium. In this study, we employed flow cytometry and histologic techniques to evaluate this process. Donor bone marrow was harvested from transgenic mice expressing the LacZ or eGFP gene ubiquitously, or under the control of the human surfactant protein (SP)-C promoter, and transplanted into lethally irradiated, neonatal mice. In recipients transplanted with marrow that express eGFP or lacZ ubiquitously, light microscopy revealed cells whose morphology and location were compatible with a type II cell phenotype. Consistent with this, fluorescent microscopy suggested colocalization of eGFP and pro–SP-C proteins in single cells. In mice transplanted with SP-C–eGFP marrow, engraftment was not detectable by histology or flow cytometry. We therefore used deconvolution microscopy to reanalyze histologic sections that were thought to show marrow-derived type II cells. We found that all putative marrow-derived pneumocytes resulted from the overlapping fluorescent signals of an endogenous pro–SP-C+ type II cell and a donor-derived eGFP+ cell. Taken together, our observations underscore the technical difficulties associated with evaluating engraftment in lung, and argue against a contributory role for marrow cells in populating the alveolar epithelium.

Key Words: bone marrow • engraftment • type II cell • stem cell




This article has been cited by other articles:


Home page
 Stem CellsHome page
J. S. Reese, J. C. Roth, and S. L. Gerson
Bone Marrow-Derived Cells Exhibiting Lung Epithelial Cell Characteristics Are Enriched In Vivo Using Methylguanine DNA Methyltransferase-Mediated Drug Resistance
Stem Cells, March 1, 2008; 26(3): 675 - 681.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Serrano-Mollar, M. Nacher, G. Gay-Jordi, D. Closa, A. Xaubet, and O. Bulbena
Intratracheal Transplantation of Alveolar Type II Cells Reverses Bleomycin-induced Lung Fibrosis
Am. J. Respir. Crit. Care Med., December 15, 2007; 176(12): 1261 - 1268.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Xu, A. Mora, H. Shim, A. Stecenko, K. L. Brigham, and M. Rojas
Role of the SDF-1/CXCR4 Axis in the Pathogenesis of Lung Injury and Fibrosis
Am. J. Respir. Cell Mol. Biol., September 1, 2007; 37(3): 291 - 299.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
J. M. Aliotta, F. M. Sanchez-Guijo, G. J. Dooner, K. W. Johnson, M. S. Dooner, K. A. Greer, D. Greer, J. Pimentel, L. M. Kolankiewicz, N. Puente, et al.
Alteration of Marrow Cell Gene Expression, Protein Production, and Engraftment into Lung by Lung-Derived Microvesicles: A Novel Mechanism for Phenotype Modulation
Stem Cells, September 1, 2007; 25(9): 2245 - 2256.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. L. Spees, D. A. Pociask, D. E. Sullivan, M. J. Whitney, J. A. Lasky, D. J. Prockop, and A. R. Brody
Engraftment of Bone Marrow Progenitor Cells in a Rat Model of Asbestos-Induced Pulmonary Fibrosis
Am. J. Respir. Crit. Care Med., August 15, 2007; 176(4): 385 - 394.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
E. L. Herzog, J. Van Arnam, B. Hu, J. Zhang, Q. Chen, A. M. Haberman, and D. S. Krause
Lung-specific nuclear reprogramming is accompanied by heterokaryon formation and Y chromosome loss following bone marrow transplantation and secondary inflammation
FASEB J, August 1, 2007; 21(10): 2592 - 2601.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
M. R. Loebinger and S. M. Janes
Stem Cells for Lung Disease
Chest, July 1, 2007; 132(1): 279 - 285.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
S. Aguilar, E. Nye, J. Chan, M. Loebinger, B. Spencer-Dene, N. Fisk, G. Stamp, D. Bonnet, and S. M. Janes
Murine but Not Human Mesenchymal Stem Cells Generate Osteosarcoma-Like Lesions in the Lung
Stem Cells, June 1, 2007; 25(6): 1586 - 1594.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
E. L. Herzog and D. S. Krause
Engraftment of Marrow-derived Epithelial Cells: The Role of Fusion
Proceedings of the ATS, November 1, 2006; 3(8): 691 - 695.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
E. L. Herzog, J. Van Arnam, B. Hu, and D. S. Krause
Threshold of lung injury required for the appearance of marrow-derived lung epithelia.
Stem Cells, August 1, 2006; 24(8): 1986 - 1992.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
S. D. Shapiro
Extracellular matrix in lung disease. Proceedings of the Twenty-First Transatlantic Airway Conference.
Proceedings of the ATS, July 1, 2006; 3(5): 397 - 455.
[Full Text] [PDF]

Home page
 DevelopmentHome page
E. L. Rawlins and B. L. M. Hogan
Epithelial stem cells of the lung: privileged few or opportunities for many?
Development, July 1, 2006; 133(13): 2455 - 2465.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
C. E. Murry, H. Reinecke, and L. M. Pabon
Regeneration Gaps: Observations on Stem Cells and Cardiac Repair
J. Am. Coll. Cardiol., May 2, 2006; 47(9): 1777 - 1785.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
B. R. Stripp and S. D. Shapiro
Stem Cells in Lung Disease, Repair, and the Potential for Therapeutic Interventions: State-of-the-Art and Future Challenges.
Am. J. Respir. Cell Mol. Biol., May 1, 2006; 34(5): 517 - 522.
[Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
D. J. Weiss, M. A. Berberich, Z. Borok, D. B. Gail, J. K. Kolls, C. Penland, and D. J. Prockop
Adult Stem Cells, Lung Biology, and Lung Disease
Proceedings of the ATS, May 1, 2006; 3(3): 193 - 207.
[Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
M. Selman and A. Pardo
Role of epithelial cells in idiopathic pulmonary fibrosis: from innocent targets to serial killers.
Proceedings of the ATS, January 1, 2006; 3(4): 364 - 372.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. M. Hajjar, H. Harowicz, H. D. Liggitt, P. J. Fink, C. B. Wilson, and S. J. Skerrett
An Essential Role for Non-Bone Marrow-Derived Cells in Control of Pseudomonas aeruginosa Pneumonia
Am. J. Respir. Cell Mol. Biol., November 1, 2005; 33(5): 470 - 475.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2005 American Thoracic Society.