Published ahead of print on April 5, 2007, doi:10.1165/rcmb.2006-0466OC
© 2007 American Thoracic Society DOI: 10.1165/rcmb.2006-0466OC Transcriptional Profiling of Mucociliary Differentiation in Human Airway Epithelial CellsClinical Research Branch, Human Studies Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park; and Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina, Chapel Hill, North Carolina Correspondence and requests for reprints should be addressed to Robert B. Devlin, U.S. Environmental Protection Agency, Clinical Research Branch, Human Studies Division, 104 Mason Farm Road, Chapel Hill, NC 27599–7315. E-mail: devlin.robert{at}epa.gov
When cultured at an air–liquid interface (ALI) in the appropriate medium, primary human airway epithelial cells form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells. This culture system provides a useful tool for the in vitro study of airway epithelial biology and differentiation. We have performed microarray analysis on ALI cultures of human bronchial epithelial cells (HBECs) grown over a 28-d period to identify genes involved in mucociliary differentiation. We identified over 2,000 genes that displayed statistically significant 2-fold or greater changes in expression during the time course. Of the genes showing the largest increases, many are involved in processes associated with airway epithelial biology, such as cell adhesion, immunity, transport, and cilia formation; however, many novel genes were also identified. We compared our results with data from proteomic analyses of the ciliary axoneme and identified candidate genes that may have roles in cilia formation or function. Gene networks were generated using Ingenuity Pathways Analysis (Ingenuity Systems, Redwood City, CA) to identify signaling pathways involved in mucociliary cell differentiation or function. Networks containing genes involved in TGF-
Key Words: bronchial epithelium • differentiation • cilia • microarrays
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