Online Supplement to: The Phagocytosis of Crystalline Silica
Particles by Macrophages
Legend for Figure 2 movie: To visualize the initial binding of crystalline silica particles
on to MH-S macrophages, images were captured every 30 seconds for 90 minutes using DIC optics
and a 63x objective. The final QuickTime movie plays at 120x real time.
Legend for Figure 3, movies 1 and 2: Confocal image stacks were collected as described
in Methods after 3 hours exposure of CMFDA labeled macrophages to TRITC-silica (movie 1)
or plain silica (movie 2). For movie 1, the data were volume rendered using Imaris (Bitplane, Inc.)
and saved as a QuickTime VR movie. The volume can be rotated in any direction by clicking
and dragging on the image. Movie 2 was made by creating a series of maximum intensity projections
of each channel while rotating the data 360 degrees with ImageJ. The two channels were merged
(red: silica, green: cytoplasmic stain) and the resulting stack was saved as a QuickTime movie.
Copyright © 2009 American Thoracic Society.
|
