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Published ahead of print on March 6, 2003, doi:10.1165/rcmb.2002-0152OC

Am. J. Respir. Cell Mol. Biol., Volume 29, Number 2, August 2003, 206-212

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Submitted on August 13, 2002
Revised on March 4, 2003

Lactoperoxidase and Human Airway Host Defense

Corinne Wijkstrom-Frei1, Souheil El-Chemaly1, Radia Ali-Rachedi2, Cynthia Gerson2, Miguel A Cobas3, Rosanna Forteza1, Matthias Salathe1, and Gregory E Conner2*

1 Division of Pulmonary and Critical Care Medicine,Department of Medicine, University of Miami School of Medicine, Miami, FL, USA, 2 Cell Biology and Anatomy, University of Miami School of Medicine, Miami, FL, USA, 3 Anesthesiology, University of Miami School of Medicine, Miami, FL, USA

* To whom correspondence should be addressed. E-mail: gconner{at}miami.edu.

The lactoperoxidase (LPO) antibiotic system is a well-characterized component of mammary and salivary gland secretions. Since LPO has been shown to function in ovine airways, human airway tissue and secretions were examined for the presence of LPO and its substrate, the anion thiocyanate (SCN-). In addition, human airway secretions were tested for LPO-mediated antibacterial activity and LPO's activity was assessed against some human airway pathogens. The data showed that normal human airway secretions contained LPO enzyme activity (0.65 ± 0.09 µg/mg secreted protein; n = 17) and Western blots of secretions demonstrated bands of the expected sizes for LPO. LPO mRNA was detected in trachea by sequencing PCR amplified cDNA. SCN-, LPO's substrate, was present in undiluted airway secretions at concentrations sufficient for LPO catalysis (0.46 ± 0.19 mM; n = 8) and diluted secretions contained antibacterial activity with LPO-like properties. Immunocytochemistry localized LPO to submucosal glands in human bronchi. Finally, as expected based on the known antibacterial spectrum of the LPO system, airway secretions showed LPO-dependent activity against Pseudomonas aeruginosa. In addition, the airway LPO system was shown to be effective against Burkholderia cepacia and Haemophilus influenzae. Thus, a functional LPO system exists in human airways and may contribute to airway host defense against infection.




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