Published ahead of print on May 14, 2003, doi:10.1165/rcmb.2002-0286OC
Am. J. Respir. Cell Mol. Biol., Volume 29, Number 4, October 2003, 523-530
A more recent version of this article appeared on October 1, 2003
Submitted on December 4, 2002
Revised on May 13, 2003
Myeloid Related Protein (MRP)-8/14 Stimulates IL-8 Production in Airway Epithelial Cells
Ali Ahmad1*, Darren L Bayley1, Shiping He1, and Robert A Stockley1
1 Department of Medicine, University of Birmingham, Birmingham, United Kingdom
* To whom correspondence should be addressed. E-mail: a.ahmad{at}bham.ac.uk.
Excessive neutrophil recruitment is implicated in the pathogenesis of chronic lung diseases by causing collateral tissue damage. The cells move from the circulation in response to chemokines, such as IL-8, that is secreted by several lung cell types including epithelial cells. This study has investigated factors present in bronchial secretions that are responsible for IL-8 expression and secretion by epithelial cells and hence initiate or perpetuate the recruitment of neutrophils.
A549 epithelial cells were stimulated with pro-inflammatory molecules likely to be of relevance in the lung. TNF- , IL-1 and LPS stimulated IL-8 production from epithelial cells in a dose and time-dependent manner and these effects were abrogated by specific antibodies or inhibitors. Bronchial secretions also stimulated IL-8 production and LPS accounted for approximately 33% of this activity. An abundant 32kDa protein capable of stimulating IL-8 production was isolated from the secretion and identified as neutrophil cytoplasmic protein MRP-14, which is the heavy polypeptide chain in the MRP-8/14 heterodimer. Abrogation of MRP-14 activity with a specific antibody also reduced the IL-8 stimulating potential of bronchial secretions suggesting it was a significant stimulus to IL-8 production in the lung and may amplify the neutrophilic inflammation seen in bronchial disease.
This article has been cited by other articles:

|
 |

|
 |
 
M. G. Ionita, A. Vink, I. E. Dijke, J. D. Laman, W. Peeters, P. H. van der Kraak, F. L. Moll, J.-P. P.M. de Vries, G. Pasterkamp, and D. P.V. de Kleijn
High Levels of Myeloid-Related Protein 14 in Human Atherosclerotic Plaques Correlate With the Characteristics of Rupture-Prone Lesions
Arterioscler Thromb Vasc Biol,
August 1, 2009;
29(8):
1220 - 1227.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Endoh, Y. M. Chung, I. A. Clark, C. L. Geczy, and K. Hsu
IL-10-Dependent S100A8 Gene Induction in Monocytes/Macrophages by Double-Stranded RNA
J. Immunol.,
February 15, 2009;
182(4):
2258 - 2268.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
P. Pouliot, I. Plante, M.-A. Raquil, P. A. Tessier, and M. Olivier
Myeloid-Related Proteins Rapidly Modulate Macrophage Nitric Oxide Production during Innate Immune Response
J. Immunol.,
September 1, 2008;
181(5):
3595 - 3601.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. F. Chung and I. M. Adcock
Multifaceted mechanisms in COPD: inflammation, immunity, and tissue repair and destruction
Eur. Respir. J.,
June 1, 2008;
31(6):
1334 - 1356.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M.-A. Raquil, N. Anceriz, P. Rouleau, and P. A. Tessier
Blockade of Antimicrobial Proteins S100A8 and S100A9 Inhibits Phagocyte Migration to the Alveoli in Streptococcal Pneumonia
J. Immunol.,
March 1, 2008;
180(5):
3366 - 3374.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. M. McCormick, F. Rahimi, Y. V. Bobryshev, K. Gaus, H. Zreiqat, H. Cai, R. S. A. Lord, and C. L. Geczy
S100A8 and S100A9 in Human Arterial Wall: IMPLICATIONS FOR ATHEROGENESIS
J. Biol. Chem.,
December 16, 2005;
280(50):
41521 - 41529.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. Lominadze, M. J. Rane, M. Merchant, J. Cai, R. A. Ward, and K. R. McLeish
Myeloid-Related Protein-14 Is a p38 MAPK Substrate in Human Neutrophils
J. Immunol.,
June 1, 2005;
174(11):
7257 - 7267.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Hsu, R. J. Passey, Y. Endoh, F. Rahimi, P. Youssef, T. Yen, and C. L. Geczy
Regulation of S100A8 by Glucocorticoids
J. Immunol.,
February 15, 2005;
174(4):
2318 - 2326.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Tugizov, J. Berline, R. Herrera, M. E. Penaranda, M. Nakagawa, and J. Palefsky
Inhibition of Human Papillomavirus Type 16 E7 Phosphorylation by the S100 MRP-8/14 Protein Complex
J. Virol.,
January 15, 2005;
79(2):
1099 - 1112.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 2003 American Thoracic Society.
|
|
|