help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published ahead of print on August 14, 2003, doi:10.1165/rcmb.2002-0311OC

Am. J. Respir. Cell Mol. Biol., Volume 30, Number 3, March 2004, 367-378

A more recent version of this article appeared on March 1, 2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2002-0311OCv1
30/3/367    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sitkauskiene, B.
Right arrow Articles by Lotvall, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sitkauskiene, B.
Right arrow Articles by Lotvall, J.

Submitted on December 20, 2002
Revised on August 14, 2003

Regulation of bone marrow and airway CD34+ eosinophils by IL-5

Brigita Sitkauskiene1, Anna-Karin Johansson2, Svetlana Sergejeva2, Samuel Lundin3, Margareta Sjostrand2, and Jan Lotvall2*

1 Respiratory Medicine and Allergology, Goteborg University, The Lung Pharmacology Group, Gothenburg, Sweden; Kaunas University of Medicine, Department of Pulmonology and Immunology, Kaunas, Lithuania, 2 Respiratory Medicine and Allergology, Goteborg University, The Lung Pharmacology Group, Gothenburg, Sweden, 3 Goteborg University, Department of Microbiology/Immunology, Gothenburg, Sweden

* To whom correspondence should be addressed. E-mail: jan.lotvall{at}mednet.gu.se.

The aim of this study was to evaluate the effect of a neutralizing anti-IL-5 monoclonal antibody (TRFK-5) on bone marrow and airway CD34+ and immature eosinophils. A focus was to determine to effect of the timing of treatment. Balb/c mice were OVA-sensitised and subsequently exposed to OVA from five to ten days via airway route. Animals were treated intraperitoneally with TRFK-5 or its isotype control (50µg) once at different time-points. Newly produced eosinophils were labelled using 5-bromo-2'-deoxyuridine (BrdU). BrdU+ and CD34+ eosinophil numbers were examined by immunocytochemistry. TRFK-5 reduced bone marrow immature eosinophils within three days. This effect was closely related to a reduction BrdU+ and CD34+ bone marrow eosinophils, and reduced numbers of blood eosinophils. However, BAL eosinophilia was not attenuated to the same degree. The effect of TRFK-5 was most prominent in the extended allergen-exposure protocol, where the treatment given in the middle of the exposure, with strongly reduced bone marrow CD34+ and immature bone marrow eosinophils, blood eosinophils as well as BAL BrdU+ eosinophils and BAL CD34+ eosinophils. These data argue that anti-IL-5 down-regulates eosinophilopoiesis within three days by action in the bone marrow, by inhibition of the early stages of eosinophil maturation from CD34+ cells.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2003 American Thoracic Society. 		  CCM abstracts