help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published ahead of print on July 10, 2003, doi:10.1165/rcmb.2003-0044OC

Am. J. Respir. Cell Mol. Biol., Volume 30, Number 3, March 2004, 280-287

A more recent version of this article appeared on March 1, 2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2003-0044OCv1
30/3/280    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fakhrzadeh, L.
Right arrow Articles by Laskin, D. L
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fakhrzadeh, L.
Right arrow Articles by Laskin, D. L

Submitted on February 11, 2003
Revised on July 8, 2003

SOD+/+ Mice are Resistant to Ozone-induced Tissue Injury and Increases in Nitric Oxide and TNF-{alpha}

Ladan Fakhrzadeh1, Jefferey D Laskin2, Carol R Gardner1, and Debra L Laskin1*

1 Toxicology and Pharmacology, Rutgers Unversity, Piscataway, NJ, USA, 2 Environmental and Occupational Health Science Institute, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, NJ, USA

* To whom correspondence should be addressed. E-mail: laskin{at}eohsi.rutgers.edu.

Reactive oxygen intermediates have been implicated in lung injury induced by inhaled irritants. The present studies utilized mice overexpressing Cu/Zn-superoxide dismutase (SOD+/+) to analyze their role in ozone-induced lung inflammation and cytotoxicity. Treatment of wild type (WT) mice with ozone (0.8 ppm, 3 h) resulted in increased bronchoalveolar lavage fluid (BALF) protein, which was maximal after 24-48 h. Significant increases in lung macrophages and 4-hydroxyalkenals (4-HAK) were also observed. In contrast, BALF protein and macrophage content and 4-HAK were at control levels in ozone treated SOD+/+ mice. There was also no evidence of peroxynitrite-mediated lung damage demonstrating that SOD+/+ mice are resistant to ozone toxicity. Whereas alveolar macrophages from WT mice produced increased amounts of nitric oxide and expressed more inducible nitric oxide synthase, phospholipase A2 and tumor necrosis factor-alpha after ozone inhalation, this was not evident in cells from SOD+/+ mice. Ozone-induced decreases in interleukin-10 were also not observed. In WT mice ozone inhalation resulted in activation of NF-{kappa}B, which regulates proinflammatory gene activity. This response was significantly reduced in SOD+/+ mice. These data demonstrate that antioxidant enzymes play a critical role in ozone-induced tissue injury and in inflammatory mediator production.




This article has been cited by other articles:


Home page
 Toxicol SciHome page
R. A. Roberts, D. L. Laskin, C. V. Smith, F. M. Robertson, E. M. G. Allen, J. A. Doorn, and W. Slikker
Nitrative and Oxidative Stress in Toxicology and Disease
Toxicol. Sci., November 1, 2009; 112(1): 4 - 16.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. Dahl, R. P. Bowler, K. Juul, J. D. Crapo, S. Levy, and B. G. Nordestgaard
Superoxide Dismutase 3 Polymorphism Associated with Reduced Lung Function in Two Large Populations
Am. J. Respir. Crit. Care Med., November 1, 2008; 178(9): 906 - 912.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. E. Lang, E. S. Williams, J. P. Mizgerd, and S. A. Shore
Effect of obesity on pulmonary inflammation induced by acute ozone exposure: role of interleukin-6
Am J Physiol Lung Cell Mol Physiol, May 1, 2008; 294(5): L1013 - L1020.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. Fakhrzadeh, J. D. Laskin, and D. L. Laskin
Ozone-induced production of nitric oxide and TNF-{alpha} and tissue injury are dependent on NF-{kappa}B p50
Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L279 - L285.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2003 American Thoracic Society. 		  ATS Coding and Billing Quarterly