Published ahead of print on September 4, 2003, doi:10.1165/rcmb.2003-0136OC
Am. J. Respir. Cell Mol. Biol., Volume 30, Number 3, March 2004, 396-402
A more recent version of this article appeared on March 1, 2004
Submitted on April 14, 2003
Revised on September 2, 2003
Apoptosis and Necrosis induced by cyclic mechanical stretching in alveolar type II cells
Stefan Hammerschmidt1*, Hartmut Kuhn1, Thomas Grasenack1, Christian Gessner1, and Hubert Wirtz1
1 Medizinische Klinik I, Universitat Leipzig, Leipzig, Germany
* To whom correspondence should be addressed. E-mail: stefan.hammerschmidt{at}t-online.de.
Alveolar type II (ATII) cells are exposed to mechanical stretch during breathing and mechanical ventilation. Increased stretch may contribute to lung injury. The influence of three stretching patterns (characterized by frequency [min-1] - increase in surface area [%]: S40-13, S60-13, S40-30) on parameters of apoptosis, necrosis and membrane integrity of in rat ATII cells was compared with that in static cultures. The S40-13 stretching pattern simulated normal breathing. The other patterns were chosen to study increased amplitude and frequency. There were no significant differences between the S40-13 group and static cultures. LDH release and early apoptotic cells were significantly increased in S60-13 and S40-30 in comparison with static cultures (LDH: 0.089±0.014µg/ml and 0.177±0.050µg/ml versus 0.050±0.011µg/ml; early apoptosis: 17±3.5% and 23±3.1% versus 9.7±1.4%) at 24h. Necrosis was significantly increased only in the S40-30 group (13±2.4% versus 6.1±0.9% in static culture at 24h). Captopril as well as L-Arginine prevented apoptosis and reduced apoptotic cells to static culture levels in the S40-30 group but did not influence necrosis and LDH release. Increased mechanical stretch may contribute to lung injury by induction of apoptosis and necrosis in ATII cells. Apoptosis induced by high amplitude mechanical stretch is prevented by captopril and L-Arginine.
This article has been cited by other articles:
|
|
|
|
 
M. E. De Paepe, S. Gundavarapu, U. Tantravahi, J. R. Pepperell, S. A. Haley, F. I. Luks, and Q. Mao
Fas-Ligand-Induced Apoptosis of Respiratory Epithelial Cells Causes Disruption of Postcanalicular Alveolar Development
Am. J. Pathol.,
July 1, 2008;
173(1):
42 - 56.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-S. Lee, Y. Wang, B. S. Maciejewski, K. Esho, C. Fulton, S. Sharma, and J. Sanchez-Esteban
Interleukin-10 protects cultured fetal rat type II epithelial cells from injury induced by mechanical stretch
Am J Physiol Lung Cell Mol Physiol,
February 1, 2008;
294(2):
L225 - L232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. D. Bland, R. Ertsey, L. M. Mokres, L. Xu, B. E. Jacobson, S. Jiang, C. M. Alvira, M. Rabinovitch, E. S. Shinwell, and A. Dixit
Mechanical ventilation uncouples synthesis and assembly of elastin and increases apoptosis in lungs of newborn mice.: Prelude to defective alveolar septation during lung development?
Am J Physiol Lung Cell Mol Physiol,
January 1, 2008;
294(1):
L3 - L14.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Hammerschmidt, H. Kuhn, C. Gessner, H.-J. Seyfarth, and H. Wirtz
Stretch-Induced Alveolar Type II Cell Apoptosis: Role of Endogenous Bradykinin and PI3K-Akt Signaling
Am. J. Respir. Cell Mol. Biol.,
December 1, 2007;
37(6):
699 - 705.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Huh, H. Fujioka, Y.-C. Tung, N. Futai, R. Paine III, J. B. Grotberg, and S. Takayama
Acoustically detectable cellular-level lung injury induced by fluid mechanical stresses in microfluidic airway systems
PNAS,
November 27, 2007;
104(48):
18886 - 18891.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-S. Jiang, L.-F. Wang, H.-C. Chou, and C.-M. Chen
Angiotensin-converting enzyme inhibitor captopril attenuates ventilator-induced lung injury in rats
J Appl Physiol,
June 1, 2007;
102(6):
2098 - 2103.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Dolinay, N. Kaminski, M. Felgendreher, H. P. Kim, P. Reynolds, S. C. Watkins, D. Karp, S. Uhlig, and A. M. K. Choi
Gene expression profiling of target genes in ventilator-induced lung injury
Physiol Genomics,
September 14, 2006;
26(1):
68 - 75.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. N. Ogawa, A. Ishizaka, S. Tasaka, H. Koh, H. Ueno, F. Amaya, M. Ebina, S. Yamada, Y. Funakoshi, J. Soejima, et al.
Contribution of High-Mobility Group Box-1 to the Development of Ventilator-induced Lung Injury
Am. J. Respir. Crit. Care Med.,
August 15, 2006;
174(4):
400 - 407.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. M. McAdams, S. B. Mustafa, J. S. Shenberger, P. S. Dixon, B. M. Henson, and R. J. DiGeronimo
Cyclic stretch attenuates effects of hyperoxia on cell proliferation and viability in human alveolar epithelial cells
Am J Physiol Lung Cell Mol Physiol,
August 1, 2006;
291(2):
L166 - L174.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. B. Geller, L. J. Fitzgerald, and C. E. King
Fission in Sea Anemones: Integrative Studies of Life Cycle Evolution
Integr. Comp. Biol.,
August 1, 2005;
45(4):
615 - 622.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Naire and O. E. Jensen
Epithelial cell deformation during surfactant-mediated airway reopening: a theoretical model
J Appl Physiol,
August 1, 2005;
99(2):
458 - 471.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Hammerschmidt, H. Kuhn, U. Sack, A. Schlenska, C. Gessner, A. Gillissen, and H. Wirtz
Mechanical Stretch Alters Alveolar Type II Cell Mediator Release toward a Proinflammatory Pattern
Am. J. Respir. Cell Mol. Biol.,
August 1, 2005;
33(2):
203 - 210.
[Abstract]
[Full Text]
[PDF]
|
|
|
Copyright © 2003 American Thoracic Society.
|
|
|
