Published ahead of print on September 25, 2003, doi:10.1165/rcmb.2003-0140OC Am. J. Respir. Cell Mol. Biol., Volume 30, Number 4, April 2004, 491-499 A more recent version of this article appeared on April 1, 2004
Submitted on April 22, 2003 Lung cells transplanted to irradiated recipients generatelymphohematopoietic progenyShinji Abe1,1 Pulmonary and Critical Care Medicine Section, University of Nebraska Medical Center, Omaha, NE, USA, 2 Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USA * To whom correspondence should be addressed. E-mail: jsharp{at}unmc.edu.
Bone marrow (stem) cells can differentiate into cells in multiple tissues, including lung. Conversely there are reports that cells of non-hematopoietic tissues (brain, muscle) can give rise to lymphohematopoietic cells. Here we show lung contains cells capable of giving rise to lymphohematopoietic cells when transplanted to irradiated recipients. Whole lung cell suspensions, lung side population (SP) cells and CD45+/- lung cells obtained from male transgenic enhanced green fluorescent protein expressing (EGFP)-mice were transplanted intravenously to total body irradiated (TBI) female mice. Green fluorescent cells were recovered from the circulation and phenotyped for their expression of lymphohematopoietic markers (CD3, CD4, CD8, B220, Gr-1 and Mac-1). Lung SP cells were composed of heterogeneous populations and had less ability to give rise to lymphohematopoietic cells than did bone marrow SP cells. Furthermore, the ability of cells from the lung of aged mice to generate lymphohematopoietic progeny was equivalent to that of cells from young mice. Cells from lung with radioprotective and lymphohematopoietic reconstituting abilities were CD45+. CD45+ cells in the lung cells have lymphohematopoietic stem/progenitor cell characteristics and this has implications for cell or gene therapy applications.
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