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Published ahead of print on June 19, 2003, doi:10.1165/rcmb.2003-0186OC

Am. J. Respir. Cell Mol. Biol., Volume 29, Number 6, December 2003, 757-770

A more recent version of this article appeared on December 1, 2003
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Submitted on May 9, 2003
Revised on June 18, 2003

Interactions of Surfactant Protein D (SP-D) with Fatty Acids

Nihal S DeSilva1, Itzhak Ofek2, and Erika C Crouch1*

1 Pathology and Immunology, Washington University School of Medicine, St.Louis, MO, USA, 2 Human Microbiology, Tel Aviv University, Tel Aviv, Israel

* To whom correspondence should be addressed. E-mail: crouch{at}path.wustl.edu.

Surfactant Protein D (SP-D) plays important roles in antimicrobial host defense, inflammatory and immune regulation, and pulmonary surfactant homeostasis. The best-characterized endogenous ligand is phosphatidylinositol; however, this lipid interaction at least in part involves the carbohydrate moiety. In this study we observed that SP-D binds specifically to saturated, unsaturated, and hydroxylated fatty acids (FA). Binding of biotinylated-SP-D to FAs or biotinylated FA to SP-D was dose-dependent, saturable, and specifically competed by the corresponding unlabeled probe. Specific binding to FA chains was also demonstrated by solution phase competition for FA binding to acrylodan-labeled fatty acid binding protein (ADIFAB), and by overlay of thin layer chromatograms with SP-D. Maximal binding to FA was dependent on calcium, and binding was localized to the neck and carbohydrate recognition domains (CRD) using recombinant trimeric neck+CRDs. Saccharide ligands showed complex, dose-dependent effects on FA binding, and FAs showed dose- and physical state-dependent effects on the binding of SP-D to mannan. In addition, CD spectroscopy suggested alterations in SP-D structure associated with binding to monomeric FA. Together, the findings indicate specific binding of FA to one or more sites in the CRD. We speculate that the binding of SP-D to the fatty acyl chains of surfactant lipids, microbial ligands, or other complex lipids contribute to the diverse biological functions of SP-D in vivo.




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