help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published ahead of print on October 30, 2003, doi:10.1165/rcmb.2003-0202OC

Am. J. Respir. Cell Mol. Biol., Volume 30, Number 5, May 2004, 710-719

A more recent version of this article appeared on May 1, 2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2003-0202OCv1
30/5/710    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Choi, K. H
Right arrow Articles by Cheng, P.-W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Choi, K. H
Right arrow Articles by Cheng, P.-W.

Submitted on May 28, 2003
Revised on October 30, 2003

Mucin Biosynthesis: Bovine C2GnT-M Gene, Tissue-Specific Expression, and Herpes Virus-4 Homologue

Kyung H Choi1, Fernando A Osorio2, and Pi-Wan Cheng3*

1 Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA, 2 Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, NE, USA, 3 Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE, USA

* To whom correspondence should be addressed. E-mail: pcheng{at}unmc.edu.

Mucin glycans are the major determinant of mucin functions. Mucin glycan branch structures, which increase structural heterogeneity and thus functional potential, are extended from {beta}6 N-acetylglucosaminides formed by {beta}6 N-acetylglucosaminyltransferases ({beta}6GnT). Core 2 {beta}6GnT-M (C2GnT-M) is the only branching enzyme that can synthesize all known mucin {beta}6 N-acetylglucosaminides. We report the cloning of four different bovine (b) C2GnT-M transcripts which are different only at 5'-untranslated regions. Two bC2GnT-M transcripts are found exclusively in tracheal epithelium and testis while the other two in all other mucus-secreting tissues. The bC2GnT-M gene contains four exons spanning 5.3 Kb and the entire open reading frame (ORF) is in one exon. The bC2GnT-M ORF has 95, 83, and 75 % sequence identity to those of bovine herpes virus type 4 (BHV-4), human, and rat C2GnT-Ms, respectively. The homology between bovine and BHV-4 C2GnT-M genes is in the region between 170 nucleotides upstream from ATG start codon and 114 nucleotides downstream from TGA stop codon of the viral gene. Localized at the non-conserved region of the viral genome, the BHV-4 C2GnT-M gene is the only known viral C2GnT-M gene. The results suggest that BHV-4 acquired its C2GnT-M gene from the bovine gene. The mechanism of the viral acquisition of bC2GnT-M gene and the roles of the C2GnT-M gene in the survival and pathogenesis of this virus remain to be elucidated.




This article has been cited by other articles:


Home page
 GlycobiologyHome page
M. Hashimoto, S. Tan, N. Mori, H. Cheng, and P.-W. Cheng
Mucin biosynthesis: Molecular cloning and expression of mouse mucus-type core 2 {beta}1,6 N-acetylglucosaminyltransferase
Glycobiology, September 1, 2007; 17(9): 994 - 1006.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Tan and P.-W. Cheng
Mucin Biosynthesis: Identification of the cis-Regulatory Elements of Human C2GnT-M Gene
Am. J. Respir. Cell Mol. Biol., June 1, 2007; 36(6): 737 - 745.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. V. Beum, H. Basma, D. R. Bastola, and P.-W. Cheng
Mucin biosynthesis: upregulation of core 2 {beta}1,6 N-acetylglucosaminyltransferase by retinoic acid and Th2 cytokines in a human airway epithelial cell line
Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L116 - L124.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
N. Markine-Goriaynoff, L. Gillet, J. L. Van Etten, H. Korres, N. Verma, and A. Vanderplasschen
Glycosyltransferases encoded by viruses
J. Gen. Virol., October 1, 2004; 85(10): 2741 - 2754.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Singh, G. A. Khan, L. Kinarsky, H. Cheng, J. Wilken, K. H. Choi, E. Bedows, S. Sherman, and P.-W. Cheng
Identification of Disulfide Bonds among the Nine Core 2 N-Acetylglucosaminyltransferase-M Cysteines Conserved in the Mucin {beta}6-N-Acetylglucosaminyltransferase Family
J. Biol. Chem., September 10, 2004; 279(37): 38969 - 38977.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2003 American Thoracic Society.