Published ahead of print on October 24, 2003, doi:10.1165/rcmb.2003-0312OC Am. J. Respir. Cell Mol. Biol., Volume 30, Number 5, May 2004, 620-626 A more recent version of this article appeared on May 1, 2004
Submitted on August 21, 2003 Monocyte recruitment into the lungs in pneumococcal pneumoniaYukinobu Goto1,1 St. Paul's Hospital, McDonald Research Laboratories and iCAPTURE Centre, University of British Columbia, Vancouver, British Columbia, Canada * To whom correspondence should be addressed. E-mail: svaneeden{at}mrl.ubc.ca.
The recruitment of monocytes into the alveolar spaces is crucial for clearing infections and resolving the inflammatory response. We have previously reported the effect of acute pneumonia on monocyte transport through the bone marrow and the present study concerns their clearance from the blood and migration into the lung air spaces. Dividing monocytes were labeled with the thymidine analog, 5'-bromo-2'-deoxyuridine (BrdU). Whole blood containing the labeled monocytes (MOBrdU) was transfused from donor rabbits either with pneumonia or from uninfected controls into recipients with pneumonia where they were detected in blood and tissues using a double immunostaining method. The results show that MOBrdU from infected animals rapidly disappeared from the circulation (P < 0.05), preferentially sequestered in the infected lung tissue within 1 h (22.0 ± 3.3% vs. 6.0 ± 0.4%, pneumonic region vs. peripheral blood, P < 0.05), and accumulated to a greater degree in pneumonic air spaces than control monocytes 48 h following transfusion (3.9 ± 0.5% vs. 1.1 ± 0.1%, P < 0.05). We conclude that immature monocytes released from the marrow by pneumonia sequester rapidly in lung microvessels but their migration in pneumonic air spaces is delayed.
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