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Published ahead of print on February 26, 2004, doi:10.1165/rcmb.2003-0345OC

Am. J. Respir. Cell Mol. Biol., Volume 31, Number 1, July 2004, 86-91

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Submitted on September 23, 2003
Revised on February 26, 2004

MUC5AC and MUC5B mucins are decreased in cystic fibrosis airway secretions

Markus O Henke1, Armin Renner2, Rudolf M Huber3, Michael C Seeds4, and Bruce K Rubin2*

1 Pulmonary Medicine, Philipps-University Marburg, Marburg, Germany; Pediatrics, Wake Forest University Health Sciences, Winston-Salem, NC, USA, 2 Pediatrics, Wake Forest University Health Sciences, Winston-Salem, NC, USA, 3 Pulmonary Medicine, Ludwig-Maximilians-University, Munich, Germany, 4 Internal Medicine/Molecular Medicine, Wake Forest University Health Sciences, Winston-Salem, NC, USA

* To whom correspondence should be addressed. E-mail: brubin{at}wfubmc.edu.

Cystic fibrosis (CF) is characterized by progressive airway obstruction. Although it has been postulated that this is due in part to mucus hypersecretion, there are no published data showing an increase in the gel forming mucins MUC5AC or MUC5B in CF secretions. We used confocal microscopy to assess the amount of mucin-like glycoprotein and DNA in CF sputum and found more mucin in bronchitis sputum and a much greater amount of DNA in CF sputum. We then used antibodies to MUC5AC and MUC5B with Western gels and dot-blot to quantify mucin in sputum from 12 patients with CF and 11 subjects without lung disease. There was a 70% decrease in MUC5B and a 93% decrease in MUC5AC in CF sputum (p<0.005 for both). We conclude that the V/V concentration of MUC5AC and MUC5B are decreased in the CF airways relative to normal mucus. This may be due to a relative increase in other components of sputum in the CF airway or to a primary defect in mucin secretion in CF.




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