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Published ahead of print on June 17, 2004, doi:10.1165/rcmb.2003-0423OC

Am. J. Respir. Cell Mol. Biol., Volume 31, Number 3, September 2004, 309-316

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Submitted on November 25, 2003
Revised on June 16, 2004

Identification of genes differentially expressed in rat alveolar type I cells

Katherine A Dahlin1, Edward M Mager1, Lennell Allen1, Zachary Tigue1, Lee Goodglick2, Madhuri Wadehra2, and Leland Dobbs3*

1 Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA, 2 Department of Medicine, University of California Los Angeles, The David Geffen School of Medicine, Los Angeles, CA, USA, 3 Department of Medicine, University of California San Francisco, San Francisco, CA, USA; Department of Pediatrics, University of California San Francisco, San Francisco, CA, USA; Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA

* To whom correspondence should be addressed. E-mail: dobbs{at}itsa.ucsf.edu.

Although approximately 98% of the internal surface area of the lung is lined by alveolar type I cells, little is known about the functions of this cell type. Using freshly isolated rat type I and type II cells, we created a subtraction library by suppression subtractive hybridization in order to identify genes differentially expressed by type I cells. We identified twelve genes of known function that are differentially expressed by type I cells. Differential expression of all 12 genes was confirmed by Northern blotting; we confirmed differential expression by immunocytochemistry for 3 genes for which suitable antibodies were available. Most of the genes code for proteins that are multifunctional. From the known functions of these genes, we infer that type I cells may play a role in the maintenance of normal alveolar homeostasis and protection from injury, lung development and remodeling, host defense, tumor/growth suppression, and surfactant metabolism, among other functions.




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