help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published ahead of print on April 15, 2004, doi:10.1165/rcmb.2004-0026OC

Am. J. Respir. Cell Mol. Biol., Volume 31, Number 3, September 2004, 257-265

A more recent version of this article appeared on September 1, 2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2004-0026OCv1
31/3/257    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by King, N. E
Right arrow Articles by Rothenberg, M. E
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by King, N. E
Right arrow Articles by Rothenberg, M. E

Submitted on January 30, 2004
Revised on April 14, 2004

Expression and regulation of a disintegrin and metalloproteinase (ADAM)8 in experimental asthma

Nina E King1, Nives Zimmermann1, Samuel M Pope1, Patricia C Fulkerson1, Nikolaos M Nikolaidis1, Anil Mishra1, David P Witte2, and Marc E Rothenberg1*

1 Division of Allergy and Immunology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA, 2 Division of Pathology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA

* To whom correspondence should be addressed. E-mail: rothenberg{at}chmcc.org.

Asthma, a complex chronic inflammatory pulmonary disorder, is on the rise despite intense ongoing research. To elucidate novel pathways involved in asthma pathogenesis, we used transcript expression profiling in a murine model of asthma. Employing asthma models induced by different allergens (ovalbumin [OVA] and Aspergillus fumigatus) we uncovered the involvement of ADAM8, a member of a metalloproteinase and disintegrin (ADAM) family. In situ hybridization of mouse lungs revealed strong ADAM8 induction in peribronchial and perivascular inflammatory cells as well as in bronchiolar epithelial cells following allergen challenge. Sequence analysis of lung ADAM8 cDNA identified a novel splice variant of ADAM8, that contained an additional exon in juxtaposition to the transmembrane domain. Allergen-induced ADAM8 mRNA accumulation in the lung was dose- and time-dependent. Transgenic or pharmacological delivery of IL-4 or IL-13 to the lungs resulted in a marked increase of ADAM8 expression. Gene-targeted mice studies revealed that OVA-induced ADAM8 was largely dependent upon STAT6 and the IL-4 receptor (R) {alpha}-chain. Thus, ADAM8 is an allergen-, IL-4, and IL-13-induced gene in the experimental asthmatic lung. Taken together with the role of ADAM33 in asthma, these results suggest that allergic lung responses involve the interplay of diverse members of the ADAM family.




This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. Estrella, N. Rocks, G. Paulissen, F. Quesada-Calvo, A. Noel, E. Vilain, P. Lassalle, I. Tillie-Leblond, D. Cataldo, and P. Gosset
Role of A Disintegrin And Metalloprotease-12 in Neutrophil Recruitment Induced by Airway Epithelium
Am. J. Respir. Cell Mol. Biol., October 1, 2009; 41(4): 449 - 458.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
R. Tachdjian, C. Mathias, S. Al Khatib, P. J. Bryce, H. S. Kim, F. Blaeser, B. D. O'Connor, D. Rzymkiewicz, A. Chen, M. J. Holtzman, et al.
Pathogenicity of a disease-associated human IL-4 receptor allele in experimental asthma
J. Exp. Med., September 28, 2009; 206(10): 2191 - 2204.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
G. Tolgyesi, V. Molnar, A. F. Semsei, P. Kiszel, I. Ungvari, P. Pocza, Z. Wiener, Z. I. Komlosi, L. Kunos, G. Galffy, et al.
Gene expression profiling of experimental asthma reveals a possible role of paraoxonase-1 in the disease
Int. Immunol., August 1, 2009; 21(8): 967 - 975.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. P. Christophi, C. A. Hudson, M. Panos, R. C. Gruber, and P. T. Massa
Modulation of Macrophage Infiltration and Inflammatory Activity by the Phosphatase SHP-1 in Virus-Induced Demyelinating Disease
J. Virol., January 15, 2009; 83(2): 522 - 539.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
N. Novershtern, Z. Itzhaki, O. Manor, N. Friedman, and N. Kaminski
A Functional and Regulatory Map of Asthma
Am. J. Respir. Cell Mol. Biol., March 1, 2008; 38(3): 324 - 336.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
D. Lu, S. Xie, M. B. Sukkar, X. Lu, M. F. Scully, and K. F. Chung
Inhibition of Airway Smooth Muscle Adhesion and Migration by the Disintegrin Domain of ADAM-15
Am. J. Respir. Cell Mol. Biol., October 1, 2007; 37(4): 494 - 500.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Gomez-Gaviro, M. Dominguez-Luis, J. Canchado, J. Calafat, H. Janssen, E. Lara-Pezzi, A. Fourie, A. Tugores, A. Valenzuela-Fernandez, F. Mollinedo, et al.
Expression and Regulation of the Metalloproteinase ADAM-8 during Human Neutrophil Pathophysiological Activation and Its Catalytic Activity on L-Selectin Shedding
J. Immunol., June 15, 2007; 178(12): 8053 - 8063.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Munitz, I. Bachelet, F. D. Finkelman, M. E. Rothenberg, and F. Levi-Schaffer
CD48 Is Critically Involved in Allergic Eosinophilic Airway Inflammation
Am. J. Respir. Crit. Care Med., May 1, 2007; 175(9): 911 - 918.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Ohtsu, P. J. Dempsey, and S. Eguchi
ADAMs as mediators of EGF receptor transactivation by G protein-coupled receptors
Am J Physiol Cell Physiol, July 1, 2006; 291(1): C1 - C10.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
N. Zimmermann, M. P. Doepker, D. P. Witte, K. F. Stringer, P. C. Fulkerson, S. M. Pope, E. B. Brandt, A. Mishra, N. E. King, N. M. Nikolaidis, et al.
Expression and Regulation of Small Proline-Rich Protein 2 in Allergic Inflammation
Am. J. Respir. Cell Mol. Biol., May 1, 2005; 32(5): 428 - 435.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. W. Johansson, M. H. Lye, S. R. Barthel, A. K. Duffy, D. S. Annis, and D. F. Mosher
Eosinophils Adhere to Vascular Cell Adhesion Molecule-1 via Podosomes
Am. J. Respir. Cell Mol. Biol., October 1, 2004; 31(4): 413 - 422.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2004 American Thoracic Society.