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Published ahead of print on March 11, 2005, doi:10.1165/rcmb.2004-0374OC

Am. J. Respir. Cell Mol. Biol., Volume 32, Number 6, June 2005, 553-559

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Submitted on November 24, 2004
Revised on March 10, 2005

Competition between elastase and related proteases from human neutrophil for binding to {alpha}1-Pi

Brice Korkmaz1, Pierre Poutrain1, Eric Hazouard1, Michele de Monte2, Sylvie Attucci1, and Francis L Gauthier1*

1 INSERM U618, Tours, France; University Francois Rabelais, Tours, France, 2 University Francois Rabelais, Tours, France; IFR 135, Tours, France

* To whom correspondence should be addressed. E-mail: gauthier{at}univ-tours.fr.

The protease-antiprotease imbalance that is characteristic of most inflammatory lung disorders depends on the spatial-temporal regulation of active inhibitor and protease concentrations in lung secretions. We have studied the competition between the three main serine proteases from human neutrophil primary granules in their binding to {alpha}1-Pi, the main serine proteases inhibitor in lung secretions. Elastase was the only target of {alpha}1-Pi when identical molar amounts of purified inhibitor and the three proteases were tested together. The other two proteases were only inhibited once elastase was saturated. Elastase remained the preferred target of inhibitors when bronchoalveolar lavage fluids from patients with lung pneumonia and acute respiratory distress syndrome were used as the source of inhibitors, in spite of the presence of additional inhibitors in lung secretions. Since neutrophil proteases are expressed at the neutrophil surface, we also measured residual activities of membrane-bound proteases after purified neutrophils were incubated with bronchoalveolar fluids. Again, elastase was the preferred target of the inhibitors. We conclude that protease 3 and cathepsin G are not controlled as efficiently as elastase in lung secretions, a feature that must be taken into account when developing inhibitor-based anti inflammatory therapies.




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