help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published ahead of print on February 10, 2006, doi:10.1165/rcmb.2004-0389OC

Am. J. Respir. Cell Mol. Biol., Volume 34, Number 6, June 2006, 727-737

A more recent version of this article appeared on June 1, 2006
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2004-0389OCv1
34/6/727    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wade, K. C
Right arrow Articles by Ballard, P. L
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wade, K. C
Right arrow Articles by Ballard, P. L

Submitted on December 7, 2004
Revised on February 8, 2006

Gene Induction during Differentiation of Human Pulmonary Type II Cells In Vitro

Kelly C Wade1, Susan H Guttentag1, Linda W Gonzales1, Kathryn L Maschhoff1, John Gonzales1, Venkatadri Kolla1, Sunil Singhal2, and Philip L Ballard1*

1 Department of Pediatrics, Division of Neonatology, University of Pennsylvania School of Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA, USA, 2 Section of Thoracic Surgery, Department of Surgery, University of Pennsylvania School of Medicine, Philadelphia, PA, USA

* To whom correspondence should be addressed. E-mail: ballardp{at}email.chop.edu.

Mature alveolar type II cells that produce pulmonary surfactant are essential for adaptation to extrauterine life. We profiled gene expression in human fetal lung epithelial cells cultured in serum-free medium containing dexamethasone and cAMP, a treatment that induces differentiation of type II cells. Microarray analysis identified 388 genes that were induced >1.5-fold by 72 h hormone treatment. Induced genes represented all categories of molecular function and subcellular location with increased frequency in the categories of ionic channel, cell adhesion, surface film, lysosome, extracellular matrix and basement membrane. In time course experiments, self organizing map analysis identified a cluster of 17 genes that were slowly but highly induced (5- to ~190-fold) and represented 4 functional categories: surfactant-related (SFTPC, SFTPA, PGC, SFTPB, LAMP3, LPL), regulatory (WIF2, IGF2, IL1RL1, NR4A2, HIF3A), metabolic (MAOA, ADH1B, SEPP1), and transport (SCNN1A, CLDN18, AQP4). Induction of both mRNA and protein for these genes, which included 9 newly identified regulated genes, was confirmed, and cellular localization was determined in both fetal and postnatal tissue. Induction of LAMP3 required both hormones and expression was localized to limiting membranes of lamellar bodies. Hormone-induced differentiation of human type II cells is associated with genome-wide increased expression of genes with diverse functions.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
V. Kolla, L. W. Gonzales, N. A. Bailey, P. Wang, S. Angampalli, M. H. Godinez, M. Madesh, and P. L. Ballard
Carcinoembryonic cell adhesion molecule 6 in human lung: regulated expression of a multifunctional type II cell protein
Am J Physiol Lung Cell Mol Physiol, June 1, 2009; 296(6): L1019 - L1030.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
P. R Provost, E. Boucher, and Y. Tremblay
Apolipoprotein A-I, A-II, C-II, and H expression in the developing lung and sex difference in surfactant lipids
J. Endocrinol., March 1, 2009; 200(3): 321 - 330.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
B. Kapitein, M. O. Hoekstra, E. H. J. Nijhuis, D. J. Hijnen, H. G. M. Arets, J. L. L. Kimpen, and E. F. Knol
Gene expression in CD4+ T-cells reflects heterogeneity in infant wheezing phenotypes
Eur. Respir. J., November 1, 2008; 32(5): 1203 - 1212.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. Fischer, L. K. Gonzales, V. Kolla, C. Schwarzer, F. Miot, B. Illek, and P. L. Ballard
Developmental regulation of DUOX1 expression and function in human fetal lung epithelial cells
Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1506 - L1514.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
V. Kolla, L. W. Gonzales, J. Gonzales, P. Wang, S. Angampalli, S. I. Feinstein, and P. L. Ballard
Thyroid Transcription Factor in Differentiating Type II Cells: Regulation, Isoforms, and Target Genes
Am. J. Respir. Cell Mol. Biol., February 1, 2007; 36(2): 213 - 225.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. M. McDevitt, L. W. Gonzales, R. C. Savani, and P. L. Ballard
Role of endogenous TGF-beta in glucocorticoid-induced lung type II cell differentiation
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L249 - L257.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2006 American Thoracic Society. 		  Tobacco