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Published ahead of print on June 16, 2005, doi:10.1165/rcmb.2005-0129OC

Am. J. Respir. Cell Mol. Biol., Volume 33, Number 4, October 2005, 335-342

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Submitted on April 8, 2005
Revised on June 16, 2005

Evidence That Bone Marrow Cells Do Not Contribute to the Alveolar Epithelium

Jacqueline C Chang1*, Ross Summer1, Xi Sun1, Kathleen Fitzsimmons1, and Alan Fine1

1 Department of Pulmonary and Critical Care, Boston University School of Medicine, Boston, MA, USA

* To whom correspondence should be addressed. E-mail: jachang{at}lung.bumc.bu.edu.

An ongoing controversy is the role of marrow cells in populating the alveolar epithelium. In this study, we employed flow cytometry and histological techniques to evaluate this process. Donor bone marrow was harvested from transgenic mice expressing the LacZ or eGFP gene ubiquitously, or under the control of the human spC promoter, and transplanted into lethally irradiated, neonatal mice. In recipients transplanted with marrow that express eGFP or lacZ ubiquitously, light microscopy revealed cells whose morphology and location were compatible with a type II cell phenotype. Consistent with this, fluorescent microscopy suggested co-localization of eGFP and pro-spC proteins in single cells. In mice transplanted with spC-eGFP marrow, engraftment was not detectable by histology or flow cytometry. We therefore used deconvolution microscopy to re-analyze histological sections that were thought to show marrow-derived type II cells. We found that all putative marrow-derived pneumocytes resulted from the overlapping fluorescent signals of an endogenous pro-spC+ type II cell and a donor-derived eGFP+ cell. Taken together, our observations underscore the technical difficulties associated with evaluating engraftment in lung, and argue against a contributory role for marrow cells in populating the alveolar epithelium.




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