Published ahead of print on March 30, 2006, doi:10.1165/rcmb.2005-0482OC Am. J. Respir. Cell Mol. Biol., Volume 35, Number 2, August 2006, 190-197 A more recent version of this article appeared on August 1, 2006
Submitted on December 27, 2005 Hydrogen Peroxide Induces Vascular Permeability via Regulation of VEGFKyung Sun Lee1,1 Department of Radiology, Chonbuk National University Medical School, Jeonju, Jeonbuk, Korea, Republic of, 2 Department of Internal Medicine, Airway Remodeling Laboratory, Research Center for Allergic Immune Diseases, Chonbuk National University Medical School, Jeonju, Jeonbuk, Korea, Republic of * To whom correspondence should be addressed. E-mail: leeyc{at}chonbuk.ac.kr.
Oxidative stress plays critical roles in initiation and/or worsening of respiratory disease process. Although reactive oxygen species (ROS) are shown to cause vascular leakage, the mechanisms by which ROS induce an increase in vascular permeability are not clearly understood. In this study, we have used a murine model to evaluate the effect of hydrogen peroxide (H2O2) to examine roles of ROS and the molecular mechanism in vascular permeability. The results have revealed that ROS levels, vascular endothelial growth factor (VEGF) expression, and hypoxia-inducible factor-1
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protein level, airway hyperresponsiveness as well as vascular permeability are increased after inhalation of H2O2. Administration of antioxidants markedly reduced plasma extravasation and VEGF levels in lungs treated with H2O2. These results indicate that ROS may modulate vascular permeability via up-regulation of VEGF expression.