help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published ahead of print on April 6, 2006, doi:10.1165/rcmb.2006-0027OC

Am. J. Respir. Cell Mol. Biol., Volume 35, Number 3, September 2006, 378-386

A more recent version of this article appeared on September 1, 2006
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2006-0027OCv1
35/3/378    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barthel, S. R
Right arrow Articles by Johansson, M. W
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barthel, S. R
Right arrow Articles by Johansson, M. W

Submitted on January 23, 2006
Revised on April 5, 2006

Dissection of the Hyper-adhesive Phenotype of Airway Eosinophils in Asthma

Steven R Barthel1, Nizar N Jarjour2, Deane F Mosher1*, and Mats W Johansson2

1 Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI, USA; Department of Medicine, University of Wisconsin-Madison, Madison, WI, USA, 2 Department of Medicine, University of Wisconsin-Madison, Madison, WI, USA

* To whom correspondence should be addressed. E-mail: dfmosher{at}facstaff.wisc.edu.

Asthma is characterized by appearance of eosinophils in the airway. Eosinophils purified from the airway 48 hours after segmental antigen challenge are described as exhibiting greater adhesion to albumin-coated surfaces via an unidentified {beta}2 integrin and increased expression of {alpha}M{beta}2 (CD11b/18) in comparison to purified blood eosinophils. We have investigated the determinants of this hyper-adhesive phenotype. Airway eosinophils exhibited increased reactivity with the CBRM1/5 anti-{alpha}M activation-sensitive antibody as well as enhanced adhesion to VCAM-1 (CD106) and diverse ligands, including albumin, ICAM-1 (CD54), fibrinogen, and vitronectin. Purified blood eosinophils did not adhere to the latter diverse ligands. Enhanced adhesion of airway eosinophils was blocked by anti-{alpha}M{beta}2. Podosomes, structures implicated in cell movement and proteolysis of matrix proteins, were larger and more common on airway eosinophils adherent to VCAM-1 when compared to blood eosinophils. Incubation of blood eosinophils with IL-5 replicated the phenotype of airway eosinophils. That is, IL-5 enhanced recognition of {alpha}M by CBRM1/5; stimulated {alpha}M{beta}2-mediated adhesion to VCAM-1, albumin, ICAM-1, fibrinogen, and vitronectin; and increased podosome formation on VCAM-1. Thus, the hyper-adhesion of airway eosinophils after antigen challenge is mediated by upregulated and activated {alpha}M{beta}2.




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
Z. Li, S. Garantziotis, W. Jia, E. N. Potts, S. Lalani, Z. Liu, Y.-W. He, W. M. Foster, and J. W. Hollingsworth
The extracellular matrix protein mindin regulates trafficking of murine eosinophils into the airspace
J. Leukoc. Biol., January 1, 2009; 85(1): 124 - 131.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. W. Johansson, E. A. B. Kelly, W. W. Busse, N. N. Jarjour, and D. F. Mosher
Up-Regulation and Activation of Eosinophil Integrins in Blood and Airway after Segmental Lung Antigen Challenge
J. Immunol., June 1, 2008; 180(11): 7622 - 7635.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
S. R. Barthel, M. W. Johansson, D. M. McNamee, and D. F. Mosher
Roles of integrin activation in eosinophil function and the eosinophilic inflammation of asthma
J. Leukoc. Biol., January 1, 2008; 83(1): 1 - 12.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. R. Barthel, D. S. Annis, D. F. Mosher, and M. W. Johansson
Differential Engagement of Modules 1 and 4 of Vascular Cell Adhesion Molecule-1 (CD106) by Integrins {alpha}4beta1 (CD49d/29) and {alpha}Mbeta2 (CD11b/18) of Eosinophils
J. Biol. Chem., October 27, 2006; 281(43): 32175 - 32187.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2006 American Thoracic Society. 		  SOTA, FL