Published ahead of print on November 1, 2006, doi:10.1165/rcmb.2006-0064OC Am. J. Respir. Cell Mol. Biol., Volume 36, Number 4, April 2007, 466-472 A more recent version of this article appeared on April 1, 2007
Submitted on February 9, 2006 Epithelial Ion Transport of Human Nasal Polyp and Paranasal Sinus MucosaMakoto Yasuda1,1 Department of Molecular Cell Physiology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan; Department of Otolaryngology-Head and Neck Surgery, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan, 2 Department of Molecular Cell Physiology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan, 3 Department of Otolaryngology-Head and Neck Surgery, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan, 4 Department of Otolaryngology-Head and Neck Surgery, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan; Department of Otolaryngology, Kyoto Second Red Cross Hospital, Kyoto, Japan, 5 Department of Molecular Cell Physiology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan; Department of Respiratory Molecular Medicine, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan * To whom correspondence should be addressed. E-mail: marunaka{at}koto.kpu-m.ac.jp.
Nasal cavity and paranasal sinus have various functions. However, little information is available on ion transport in these upper airway epithelia. In the present study, we measured the anion secretion and the anion channel activity to characterize the ion transport in epithelial cells prepared from human paranasal sinus mucosa (PSM) and nasal polyp (NP). To estimate the anion secretion and the anion channel activity, we measured the short-circuit current (Isc) and the transepithelial conductance (Gt) sensitive to NPPB (a Cl- channel blocker). The NPPB-sensitive Isc in PSM was larger than that in NP, correlating to the NPPB-sensitive Gt (Cl- channel activity). Forskolin stably elevated the NPPB-sensitive Isc associated with an increase in the NPPB-sensitive Gt in PSM and NP. UTP transiently stimulated the Isc associated with an elevation of Gt in PSM and NP. The stimulatory action of UTP on Isc and Gt was diminished by application of NPPB but not benzamil in PSM and NP, suggesting that UTP induced the NPPB-sensitive Isc (Cl- secretion) and Gt (Cl- channel activity). These observations suggest that in human PSM and NP cAMP stably stimulates anion secretion by activating the Cl- (anion) channels, and that UTP just transiently elevates anion secretion via activation of some Cl- (anion) channels.
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