Eosinophils represent one of the main effector cell populations of allergic airway inflammation and allergic bronchial asthma. Their infiltration correlates with many characteristics of the disease including AHR and increased mucus production. CCR-3 is the principle chemokine receptor involved in eosinophil attraction into inflamed tissue. Therefore, antagonizing CCR-3 could be a novel promising approach towards asthma therapy. We investigated the effect of a small molecular weight CCR-3 antagonist on established airway inflammation in a chronic model of experimental bronchial asthma. For this purpose ovalbumin (OVA) -i.p. sensitized BALB/c mice were chronically challenged with OVA aerosol in order to induce chronic airway inflammation and airway remodelling. The effect of antagonizing CCR-3 on asthma pathology was examined in BAL and lung histology. Airway reactivity was assessed by head-out body-plethysmography. Treatment with the CCR-3 antagonist resulted in a marked reduction of eosinophils in the broncho-alveolar lumen and in airway wall tissue whereas infiltration of lymphocytes or macrophages remained unchanged. The reduction in eosinophil infiltration was accompanied by normalization of AHR and prevention of goblet cell hyperplasia, indicating reduced mucus production. Furthermore, antagonizing CCR-3 prevented airway remodelling as defined by subepithelial fibrosis and increased accumulation of myofibrocytes in the airway wall of chronically challenged mice. These data demonstrate that antagonism of CCR3 reduces eosinophil numbers which is accompanied by diminution of asthma pathology in a mouse model of established chronic experimental asthma. Therefore, antagonizing CCR-3 represents a new approach towards a promising asthma therapy.