help button home button
AJRCMB
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Berin, M. C.
Right arrow Articles by Kagnoff, M. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Berin, M. C.
Right arrow Articles by Kagnoff, M. F.

Am. J. Respir. Cell Mol. Biol., Volume 24, Number 4, April, 2001 382-389

Regulated Production of the T Helper 2-Type T-Cell Chemoattractant TARC by Human Bronchial Epithelial Cells In Vitro and in Human Lung Xenografts

M. Cecilia Berin, Lars Eckmann, David H. Broide, and Martin F. Kagnoff

Laboratory of Mucosal Immunology, Department of Medicine, University of California, San Diego, La Jolla, California

The chemokine TARC is a ligand for the chemokine receptor CCR4 expressed on T helper (Th)2-type CD4 T cells. Allergic airway inflammation is characterized by a local increase in cells secreting Th2-type cytokines. We hypothesized that bronchial epithelial cells may be a source of chemokines known to chemoattract Th2 cells. Regulated TARC expression was studied using normal human bronchial epithelial cells and a human lung xenograft model. TARC expression was increased in normal human bronchial epithelial cells in response to tumor necrosis factor-alpha stimulation, and further upregulation of TARC was observed with interferon (IFN)-gamma but not interleukin (IL)-4 costimulation. TARC functions as a nuclear factor (NF)-kappa B target gene, as shown by the abrogation of TARC expression in response to proinflammatory stimuli when NF-kappa B activation is inhibited. In an in vivo model, minimal constitutive TARC expression was observed in human lung xenografts. Consistent with our findings in vitro, TARC messenger RNA (mRNA) expression was upregulated in the xenografts in response to IL-1, and costimulation with IFN-gamma but not IL-4 further increased TARC mRNA and protein expression. In addition, bronchoalveolar lavage fluid from asthmatic subjects after allergen challenge contained significantly increased levels of TARC, suggesting that TARC production by bronchial epithelial cells may play a role in the pathogenesis of allergic asthma.

Abbreviations: bronchoalveolar lavage, BAL; BAL fluid, BALF; complementary DNA, cDNA; enzyme-linked immunosorbent assay, ELISA; interferon, IFN; immunoglobulin, Ig; interleukin, IL; monocyte chemotactic protein, MCP; messenger RNA, mRNA; nuclear factor, NF; normal human bronchial epithelial, NHBE; phosphate-buffered saline, PBS; polymerase chain reaction, PCR; recombinant human, rh; reverse transcriptase, RT; T helper, Th; tumor necrosis factor, TNF.




This article has been cited by other articles:


Home page
 Arch Otolaryngol Head Neck SurgHome page
M. Nonaka, N. Ogihara, A. Fukumoto, A. Sakanushi, R. Pawankar, and T. Yagi
Combined Stimulation of Nasal Polyp Fibroblasts With Poly IC, Interleukin 4, and Tumor Necrosis Factor {alpha} Potently Induces Production of Thymus- and Activation-Regulated Chemokine
Arch Otolaryngol Head Neck Surg, June 1, 2008; 134(6): 630 - 635.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
E. A. Jacobsen, S. I. Ochkur, R. S. Pero, A. G. Taranova, C. A. Protheroe, D. C. Colbert, N. A. Lee, and J. J. Lee
Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells
J. Exp. Med., March 17, 2008; 205(3): 699 - 710.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
O. Tliba and Y. Amrani
Airway Smooth Muscle Cell as an Inflammatory Cell: Lessons Learned from Interferon Signaling Pathways
Proceedings of the ATS, January 1, 2008; 5(1): 106 - 112.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. M. Monick, L. S. Powers, I. Hassan, D. Groskreutz, T. O. Yarovinsky, C. W. Barrett, E. M. Castilow, D. Tifrea, S. M. Varga, and G. W. Hunninghake
Respiratory Syncytial Virus Synergizes with Th2 Cytokines to Induce Optimal Levels of TARC/CCL17
J. Immunol., August 1, 2007; 179(3): 1648 - 1658.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
I. H. Heijink, P. M. Kies, H. F. Kauffman, D. S. Postma, A. J. M. van Oosterhout, and E. Vellenga
Down-Regulation of E-Cadherin in Human Bronchial Epithelial Cells Leads to Epidermal Growth Factor Receptor-Dependent Th2 Cell-Promoting Activity
J. Immunol., June 15, 2007; 178(12): 7678 - 7685.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
I. H. Heijink, P. Marcel Kies, A. J. M. van Oosterhout, D. S. Postma, H. F. Kauffman, and E. Vellenga
Der p, IL-4, and TGF-beta Cooperatively Induce EGFR-Dependent TARC Expression in Airway Epithelium
Am. J. Respir. Cell Mol. Biol., March 1, 2007; 36(3): 351 - 359.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. Jaradat, C. Stapleton, S. L. Tilley, D. Dixon, C. J. Erikson, J. G. McCaskill, H. S. Kang, M. Angers, G. Liao, J. Collins, et al.
Modulatory Role for Retinoid-related Orphan Receptor {alpha} in Allergen-induced Lung Inflammation
Am. J. Respir. Crit. Care Med., December 15, 2006; 174(12): 1299 - 1309.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. Soler, T. R. Chapman, L. R. Poisson, L. Wang, J. Cote-Sierra, M. Ryan, A. McDonald, S. Badola, E. Fedyk, A. J. Coyle, et al.
CCR8 Expression Identifies CD4 Memory T Cells Enriched for FOXP3+ Regulatory and Th2 Effector Lymphocytes
J. Immunol., November 15, 2006; 177(10): 6940 - 6951.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. H. Heijink, E. Vellenga, J. Oostendorp, J. G. R. de Monchy, D. S. Postma, and H. F. Kauffman
Exposure to TARC alters {beta}2-adrenergic receptor signaling in human peripheral blood T lymphocytes
Am J Physiol Lung Cell Mol Physiol, July 1, 2005; 289(1): L53 - L59.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. J. Youn, M. Miller, K. J. Baek, J. W. Han, J. Nayar, S. Y. Lee, K. McElwain, S. McElwain, E. Raz, and D. H. Broide
Immunostimulatory DNA Reverses Established Allergen-Induced Airway Remodeling
J. Immunol., December 15, 2004; 173(12): 7556 - 7564.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
C. Jakubzick, H. Wen, A. Matsukawa, M. Keller, S. L. Kunkel, and C. M. Hogaboam
Role of CCR4 Ligands, CCL17 and CCL22, During Schistosoma mansoni Egg-Induced Pulmonary Granuloma Formation in Mice
Am. J. Pathol., October 1, 2004; 165(4): 1211 - 1221.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. G. Cronshaw, C. Owen, Z. Brown, and S. G. Ward
Activation of Phosphoinositide 3-Kinases by the CCR4 Ligand Macrophage-Derived Chemokine Is a Dispensable Signal for T Lymphocyte Chemotaxis
J. Immunol., June 15, 2004; 172(12): 7761 - 7770.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. F. Chong, J.-E. Murphy, T. S. Kupper, and R. C. Fuhlbrigge
E-Selectin, Thymus- and Activation-Regulated Chemokine/CCL17, and Intercellular Adhesion Molecule-1 Are Constitutively Coexpressed in Dermal Microvessels: A Foundation for a Cutaneous Immunosurveillance System
J. Immunol., February 1, 2004; 172(3): 1575 - 1581.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. S. Faffe, T. Whitehead, P. E. Moore, S. Baraldo, L. Flynt, K. Bourgeois, R. A. Panettieri, and S. A. Shore
IL-13 and IL-4 promote TARC release in human airway smooth muscle cells: role of IL-4 receptor genotype
Am J Physiol Lung Cell Mol Physiol, October 1, 2003; 285(4): L907 - L914.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
K. Honda, M. Arima, G. Cheng, S. Taki, H. Hirata, F. Eda, F. Fukushima, B. Yamaguchi, M. Hatano, T. Tokuhisa, et al.
Prostaglandin D2 Reinforces Th2 Type Inflammatory Responses of Airways to Low-dose Antigen through Bronchial Expression of Macrophage-derived Chemokine
J. Exp. Med., August 18, 2003; 198(4): 533 - 543.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Nishihara, S. Kizaka-Kondoh, P. A. Insel, and L. Eckmann
Inhibition of apoptosis in normal and transformed intestinal epithelial cells by cAMP through induction of inhibitor of apoptosis protein (IAP)-2
PNAS, July 22, 2003; 100(15): 8921 - 8926.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Y. Vermaelen, D. Cataldo, K. Tournoy, T. Maes, A. Dhulst, R. Louis, J.-M. Foidart, A. Noel, and R. Pauwels
Matrix Metalloproteinase-9-Mediated Dendritic Cell Recruitment into the Airways Is a Critical Step in a Mouse Model of Asthma
J. Immunol., July 15, 2003; 171(2): 1016 - 1022.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. A. Carey, D. R. Germolec, J. A. Bradbury, R. A. Gooch, M. P. Moorman, G. P. Flake, R. Langenbach, and D. C. Zeldin
Accentuated T Helper Type 2 Airway Response after Allergen Challenge in Cyclooxygenase-1-/- but Not Cyclooxygenase-2-/- Mice
Am. J. Respir. Crit. Care Med., June 1, 2003; 167(11): 1509 - 1515.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
T.F. Leung, C.K. Wong, C.W.K. Lam, A.M. Li, W.K. Ip, G.W.K. Wong, and T.F. Fok
Plasma TARC concentration may be a useful marker for asthmatic exacerbation in children
Eur. Respir. J., April 1, 2003; 21(4): 616 - 620.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
E. Miyazaki, S.-i. Nureki, T. Fukami, T. Shigenaga, M. Ando, K. Ito, H. Ando, K. Sugisaki, T. Kumamoto, and T. Tsuda
Elevated Levels of Thymus- and Activation-regulated Chemokine in Bronchoalveolar Lavage Fluid from Patients with Eosinophilic Pneumonia
Am. J. Respir. Crit. Care Med., April 15, 2002; 165(8): 1125 - 1131.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. C. Berin, M. B. Dwinell, L. Eckmann, and M. F. Kagnoff
Production of MDC/CCL22 by human intestinal epithelial cells
Am J Physiol Gastrointest Liver Physiol, June 1, 2001; 280(6): G1217 - G1226.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Crit. Care Med.
Copyright © 2001 American Thoracic Society.