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Role of Surfactant Protein-A in Nitric Oxide Production and Mycoplasma Killing in Congenic C57BL/6 Mice

Departments of Anesthesiology, Genomics and Pathobiology, and Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama

Address correspondence to: Dr. Sadis Matalon, Department of Anesthesiology, University of Alabama at Birmingham, UAB Dept. of Anesthesiology, 1530 3rd Ave. South, Birmingham, AL 35294-2172. E-mail: Sadis.Matalon{at}ccc.uab.edu

We generated congenic surfactant protein A (SP-A)–deficient (SP-A[-/-]) mice on the mycoplasma resistant C57BL/6 background (B6.SP-A[-/-]) and characterized their response to mycoplasma infection in comparison to C57BL/6 (B6) mice. B6.SP-A(-/-) mice infected with 10⁶ colony-forming units (cfu) of Mycoplasma pulmonis had significantly higher bacterial lung loads than B6 mice at 72 h postinfection (p.i.). At the higher infection dose of 10⁷, B6.SP-A(-/-) mice had significantly higher lung cfu at 24 h; however, no difference in mycoplasma cfu was observed between B6 and B6.SP-A(-/-) mice at 48 and 72 h p.i. We found that uninfected B6 mice had lower bronchoalveolar lavage nitrite (NO₂^-) and nitrate (NO₃^-) levels as compared with B6.SP-A(-/-) mice. On the other hand, infection of B6 mice with mycoplasmas resulted in significantly higher bronchoalveolar lavage NO₂^- and NO₃^- as compared with B6.SP-A(-/-) mice. These data indicate that SP-A may help regulate NO production in response to a specific stimulus, i.e., suppression of NO in the absence of bacteria and increased NO in the presence of bacteria. These data indicate that the contribution of SP-A to mycoplasma killing may be limited to lower doses of pathogens.

Abbreviations: alveolar macrophage, AM • acidophilic macrophage pneumonia, AMP • bronchoalveolar lavage, BAL • colony-forming unit, cfu • interferon-, IFN- • interleukin-1ß, IL-1ß • monocyte chemotactic protein, MCP • nitric oxide, NO • polymerase chain reaction, PCR • postinfection, p.i. • polymorphonuclear cells, PMN • surfactant protein A, SP-A • tumor necrosis factor-, TNF-

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