Cover: PNE were cultured on permeable membranes at an air-medium interface, exposed to air or 0.3 ppm ozone for 3 h, and prepared for en face immunofluorescent staining with DAPI (blue, nuclei) and Texas red linked to anti-tenascin-C antibody, F9A5 (extracellular tenascin-C). Red fluorescence showed tenascin-C on the denuded portions of the membrane only in cultures exposed to ozone. Air (a), ozone (b, c, d). Secondary antibody only (c). Bar = 100 mm. For further information, see article by Potter-Perigo and colleagues, 471.
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