Cover: Effect of thrombin stimulation on EC MLCK intracellular localization. BPAEC were treated with either vehicle (A), 100 nM thrombin for 2 min (B), or 100 nM thrombin for 10 min (C ), and fixed with paraformaldehyde. Fixed, permeabilized cells were imaged by confocal microscopy after immunostaining with primary antibody to MLCK (V-368), post-staining with FITC-antirabbit IgG (green), and staining with rhodamine phalloidin (red ) to assess F-actin binding. As noted in B and C, thrombin induced a rapid increase in F-actin staining and a time-dependent increase in the colocalization of MLCK and actin, as indicated by the shift in MLCK with staining from predominantly green in the control cells to yellow (mixture of the red and green fluorescence) after thrombin challenge. For further information, see the article by Verin and colleagues 767.
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