Cover: Immunofluorescence visualization of fibronectin coating on fiber surfaces done with confocal microscope and shown in pseudocolors. The color code of fluorescence intensity is indicated on the color bar to the right (scaled from 0 [bottom, blue to black] to 255 [top, white]). Top left: Fibronectin-treated asbestos fibers (2× wash) appear bright white, indicating the presence of fibronectin coating. Top right: Fibronectin-treated glass fibers (2× wash) also show fibronectin on the surface. Bottom left: Fibronectin-treated asbestos fibers washed 10 times still appear bright white, indicating the presence of fibronectin coating after 10 washings. Bottom right: Fibronectin-treated glass fibers washed 10 times show loss of fluorescence intensity. Bars show 10 µm. For more information, see article by Tsuda and colleagues 455.
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