Cover: Neuregulin (NRG)-1 stimulates pulmonary epithelial cell proliferation. Explant cultures of human fetal lungs were cultured in the presence of 10⁸ M NRG-1 for 4 d (upper panel ) or with no stimulation (lower panel ) (original magnification: ×400). During the final 24 h of culture, the explants were incubated with bromodeoxyuridine (BrdU) (2 mM), then fixed, sections cut (5 µm), and BrdU immunoreactivity detected. The lumens of prealveolar ducts are indicated with an "L"; the connective tissue between the ducts is indicated with a "CT"; and the closed arrows indicate the BrdU-labeled epithelial cells, whereas open arrows point to unlabeled epithelial cells. Two separate experiments were performed using tissue from different donors. For more information, see article by Patel and colleagues 432.
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