Cover: Immunohistochemical analysis of the lungs harvested on Day 28 after bleomycin treatment. (Top left) Intense epimorphin immunoreactivity is observed in areas of fibrosis. (Top right) Keratin labeling is seen in regenerating alveolar epithelial cells surrounding the fibrotic lesions. (Bottom left) Double-labeled immunofluorescent image clearly showing the presence of epimorphin within a fibrotic lesion (green) as well as in surrounding keratin (red)-positive regenerating epithelial cells (arrowheads). (Bottom right) The same view of previously discussed panel, using Nomarski optics, clearly shows the intra-alveolar fibrotic lesions with three-dimensional shapes and matching the colors of the panel to its left. Nuclei are shown as 4,6-diamidino-2-phenylindole (DAPI)-positive (blue). Asterisks denote intra-alveolar fibrosis. All bars = 18 µm. For more information see article by Terasaki 168.
[Table of Contents]