Cover: Human nasal polyp epithelial cells (NHNP) and 9HTEo cells imaged by confocal microscopy. (Top panels) P. aeruginosa association with monolayers. NHNP and 9HTEo cells grown on semipermeable supports are shown after exposure to 1 × 10⁸ colony-forming units/ml of PAO1/green fluorescent protein for 3 h and imaged 8 µm from the apical surface. The NHNP cells were also treated with anti-ZO-1 and a fluorescent Cy-5 tag to delineate the cell boundaries. The 9HTEo cells were treated with antifodrin, which was visualized with Cy-5. Original magnification: ×100. (Lower panels) Demonstration of tight junctions. Monolayers grown under the same conditions as shown in top panels were treated with antibody to ZO-1, a marker for tight junctions. The images shown were 3 µm from the apical surface. Original magnification: ×100. For more information, see article by Rajan and colleagues 304.
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