Cover: Mutants lacking the juxtamembrane positive charges are retained in the ER. At 24 h after transfection of CHO cells with WT his-proSP-C (A-C) or KR^11,12QQ (D-F) the cells were fixed and double-immunostained for his tag and BiP. His tag-Alexa 568-specific fluorescence is depicted in A and D; BiP-Alexa 488-specific fluorescence is depicted in B and E; and an overlay of the fluorescence by the two fluorophores is depicted in C and F. Colocalization appears as yellow. For more information see article by ten Brinke and colleagues beginning on page 156.
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