Cover: Confocal projections of left lobes at E12 cultured for 5 d. (F ) Increased amount of PGP 9.5-positive (green) nerves and many large ganglia (arrows) cover the -actin-positive cells (red) outside the proximal part of the lobe in response to GDNF. (G) Increased amount of neural tissue in response to GDNF. Lobe surrounded by p75^NTR-positive nerves and migrating neuronal precursors that have formed ganglia. (H ) Enlargement of area in G showing immunoreactivity to p75^NTR (H, red), PGP 9.5 (H ', green), and the two images merged (H ). Yellow represents colocalization, but the true relationship between the two markers can only be discerned at a higher power. (I, I ', and I  ) Single optical section through a ganglion in H (boxed area) double stained for p75^NTR and PGP 9.5, showing p75^NTR-positive membranes (I, red) around PGP 9.5-positive cytoplasm and nucleus (I ', green). The nucleoli are unstained. (I  ) The merged images show the relationship of the markers. A yellow color means that they are colocalized in that particular area which especially occurs in the nerves (arrows). For more information see article by Tollett and colleagues beginning on page 420.
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