Cover: Immuno-phenotype of granuloma-associated cells. Frozen sections of nonimmunized or PPD-immunized mice on Days 1 or 3 after the intravenous injection of PPD-coated beads were immunolabeled for MHC class II (a-d), CD11c (e-h), macrophage antigen (i-l), and CD4 (m-p). In nonimmunized mice on Day 1, the beads were surrounded by a limited number of inflammatory cells (a, e, i, and m). By contrast, on Day 1 in immunized mice, PPD-coated beads were surrounded by a mixture of inflammatory cells (c, g, k, and o) that included MHC II⁺ cells with dendritic profiles (c), CD11c⁺ DCs (g), MOMA2⁺ macrophages (k), CD4⁺ (o), and CD8⁺ T cells (not shown). By Day 3, granulomas in both nonimmunized and immunized mice contained increased numbers of MHC II⁺ cells with dendritic shapes (b and d) and MOMA2⁺ macrophages (j and l). Intensely stained CD11c⁺ DCs (f and h), CD4⁺ (n and p), and CD8⁺ (not shown) T cells, however, were more numerous in the granulomas of immunized than in nonimmunized mice. Scale bar: 100 µm. For more information see article by Iyonaga and colleagues beginning on page 671.
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