Cover: Immunostaining of human lung fibroblasts shows TIMP-3 downregulation upon transient transfection with a SRF-DsRed2 plasmid construct. TIMP-3 was immunodetected using a fluorescein isothiocyanate-conjugated secondary antibody (green fluorescence). Cells expressing SRF-DsRed2 fusion protein are easily identified by their red-orange fluorescent nucleus. SRF-transfected cells show either traces or absence of TIMP-3 in their cytoplasm, whereas untransfected cells in the same field are positive for TIMP-3 (three left upper panels). Normaski image showing the profile of all cells in the field is presented in corresponding right panels. Immunostaining with anti-TIMP-1 and anti-TIMP-2 antibodies shows no changes in these two TIMPs after SRF-DsRed2 fusion plasmid transfection (lowest two panels). Magnification bar: 20 {mu}m. For more information see article by Zhe and coworkers beginning on page 504.
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