Cover: ADAM33 mRNA expression in intact tissue by in situ hybridization. Archival tissues were screened for ß-actin mRNA to ensure intact RNA. Adjacent serial sections were hybridized to ADAM33 antisense and sense probes and subsequently stained with hematoxylin and eosin. The probes recognized a 540-bp fragment corresponding to nucleotides 2,341 to 2,880 in the 3´UTR of ADAM33. (G) Asthma lung from an 18-yr-old female with asthma who died of respiratory failure, hematoxylin and eosin stained, 10×. (H) Tissue as in (G), showing submucosal smooth muscle and epithelial layer, ADAM33 antisense probe, 60×.(I) Asthma lung from a 13-yr-old male with asthma who died of respiratory failure showing submucosal smooth muscle, ADAM33 antisense probe, 60×. Arrowhead indicates representative smooth muscle cell with ADAM33 specific hybridization. (J) Duodenum, ulcer granulation tissue (surgical) from a 62-yr-old male showing smooth muscle cells, ADAM33 antisense probe, 60×; Arrowheads indicate representative smooth muscle cells with ADAM33 specific hybridization. (K) Tissue as in (J), showing ulcer base containing macrophages (yellow arrowhead) and reactive fibroblasts (green arrowhead), ADAM33 antisense probe, 60×; (L) tissue as in (K), ADAM33 sense probe, 60×; macrophages are indicated by yellow arrowhead. Scale is indicated on each panel. For more information see article by Umland and colleagues beginning on page 571.