Immunohistochemical detection of protein kinase C(PKC) in normal lung fibroblasts (NLF) and scleroderma lung fibroblasts (SLF). NLF (A-C, G-I) and SLF (D-F, J-L) were cultured on 4-well glass chamber slides. Wild-type PKC was overexpressed in NLF (H) and SLF (K); antisense oligodeoxynucleotide treatment inhibited PKC expression in NLF (I) and SLF (L). Cells were then fixed, permeabilized, and incubated with rabbit anti-PKC(A, D, H, I, K, L), mouse monoclonal anti--smooth muscle actin (ASMA) (B, E), rabbit anti-PKC (G, J), or both rabbit anti-PKC and mouse monoclonal anti-ASMA (C, F). Following incubation with Cy2-labeled goat anti-rabbit IgG antibodies and/or Cy3-labeled goat anti-mouse IgG antibodies, staining patterns were detected by fluorescent microscopy. For more information, see article by Tourkina and colleagues beginning on page 28.
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